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茶树愈伤诱导及不同儿茶素含量细胞系筛选
刘亚军,王正荣,王婕,杨冬青,郭丽丽,孙美莲,王云生,高丽萍,夏涛
0
(安徽农业大学生命科学学院,合肥 230036;枣庄学院化学化工与材料科学学院,枣庄 277160;安徽农业大学教育部茶叶生物化学与生物技术重点实验室,合肥 230036)
摘要:
茶树组织培养是开展茶树次生代谢及调控研究重要的研究平台。在建立茶树愈伤组织培养体系的基础上,开展了茶树含不同儿茶素含量的细胞系筛选方法的研究。结果表明,在H、S和B 3种诱导培养基中,只有B培养基适合于诱导生长迅速、组织疏松、质地均一且具有一定儿茶素合成能力的愈伤组织。从外植体方面考虑,种子诱导出的愈伤组织具有较强的植株再生能力;幼茎和叶片诱导出的愈伤组织,其生长速度快、组织疏松,但再生能力弱。在此基础上,采用目视法和二分法,结合HPLC分析,对B培养基诱导的细胞系“yunjing63Y”进行了筛选,得到含不同儿茶素含量的细胞系“yunjing63Y”和“yunjing63X”。分析结果显示,细胞系“yunjing63Y”和“yunjing63X”的儿茶素组分与鲜叶相似,且二者儿茶素含量(干重)差异较大,前者为7.77 mg·g-1,后者为0.13 mg·g-1
关键词:  茶树  组织培养  儿茶素  细胞系
DOI:10.13610/j.cnki.1672-352x.20140620.016
基金项目:国家自然科学基金(31170647, 31170282, 31270730, 31300577), 高等学校博士学科点专项科研基金(KJ2012A110), 安徽省自然科学基金(1408085QC51)和安徽省高校自然科学基金(KJ2012A110)共同资助。
The induction of tea callus and screening for tea cell line containing different catechin contents
LIU Yajun,WANG Zhengrong, WANG Jie,YANG Dongqing,GUO Lili,SUN Meilian,WANG Yunsheng,GAO Liping,XIA Tao
(School of Life Science, Anhui Agricultural University, Hefei 230036;;Department of Chemical Engineering and Materials Science, Zaozhuang University, Zaozhuang 277160;;Key Lab of Tea Biochemistry and Biotechnology, Ministry of Education, Anhui Agricultural University, Hefei 230036)
Abstract:
The callus culture of tea plant provides a good platform for the regulation and metabolism of secondary metabolites in the tea plant. This research carried out the work in screening for tea cell lines containing different catechin contents based on the callus culture of the tea plant. The results showed that among H, S and B induction mediums, only B medium was fitted for inducing and screening callus which were quickly grown, loosely organized, even textured and produced a good amount of catechins. Callus induced from tender stems and leaves grew quickly and loosely, but its regeneration ability to plants was weak. Cell lines “yunjing63Y”, “yunjing63X” with different catechin contents were obtained by the visual method, dichotomy, and HPLC analysis. The results showed that catechin components in the two cell lines were similar to those in the fresh tea leaves, but the catechin contents in cell lines “yunjing63Y”and “yunjing63X” differed obviously. The catechin contents (DW) of the former was 7.77 mg·g-1, and the latter was 0.13 mg·g-1.
Key words:  tea plant (Camellia sinensis)  callus culture  catechins  cell lines

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