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盐碱地中1株硝化细菌的分离、鉴定及其硝化能力的分析
陆洪省,赵晓舒,王亚舒,张如玉,刘月月
0
(山东科技大学化学与环境工程学院,青岛 266590)
摘要:
从黄河三角洲盐碱地土壤中分离到一株异养高效硝化细菌,对该菌株外部形态、生理生化性质、16S rDNA序列、硝化能力等方面进行分析。结果表明,该菌株为革兰氏阴性菌、杆状、能以葡萄糖、蔗糖等为碳源生长,最适生长pH为9;16S rDNA基因序列分析表明,该菌株与Pseudomonas putida(D85992)的同源性达99%。根据上述结果,该菌株可鉴定为恶臭假单胞菌,并命名为Pseudomonas SKDP-1。以氨氮(NH4+-N)为惟一氮源,分别以柠檬酸钠、葡萄糖和蔗糖为碳源条件下,其氨氮转化率分别为49.5%、34%和32.8%,表明菌株Pseudomonas sp. SKDP-1在盐碱地土壤中对氮的循环方面具有良好的应用前景。
关键词:  黄河三角洲盐碱地  硝化细菌  恶臭假单胞菌  氨氮转化率
DOI:
投稿时间:2013-06-26
基金项目:山东省博士后创新项目专项基金一等(201201008)资助。
Isolation and identification of a heterotrophic nitrifying strain from saline-alkali soil and analysis of its nitration abilities
LU Hongsheng,ZHAO Xiaoshu,WANG Yashu,ZHANG Ruyu,LIU Yueyue
(School of Chemistry and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266590)
Abstract:
A heterotrophic nitrifying strain was isolated from Yellow River Delta saline-alkali soil, and its morphology, physiochemical properties, 16S rDNA sequence, and ammonia nitrogen degradation ability were examined. The results showed that this bacteria is rod, gram-negative, can use glucose, sucrose as carbon source for growth, and its optimum pH is 9. Its 16S rDNA sequence indicated 99% similarity with Pseudomonas putida(D85992). Based on the above results, this strain was believed to be a member of genus Pseudomonas and named Pseudomonas sp. SKDP-1. This strain showed high ammonia nitrogen degrading abilities of 49.5%, 34% and 32.8% with sodium citrate, glucose and sucrose as its carbon sources, respectively, and suggesting SKDP-1 has a good application prospect for nitrogen cycle in saline-alkali soil.
Key words:  Yellow River Delta saline-alkali soil  nitrobacteria  Pseudomonas putida  ammonia nitrogen degradation

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