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68份小麦脂肪氧化酶活性和戊聚糖含量的测定和聚类分析
王慧,崔文礼,张从合,顾明超,吴萍,郑文寅,张文明,姚大年
0
(安徽农业大学农学院,合肥 230036; 安徽荃银高科种业股份有限公司,合肥 230088)
摘要:
脂肪氧化酶(LOX)活性和戊聚糖是影响小麦营养和加工品质的重要因素。选用68个小麦品种(系)作为试验材料,测定其籽粒LOX活性、总戊聚糖、水溶性和非水溶性戊聚糖含量,并进行方差分析。结果表明,LOX活性、总戊聚糖、水溶性和非水溶性戊聚糖含量在品种间差异均达到极显著,LOX活性和3种戊聚糖含量的平均值分别为10.35 nkat·g-1、4.49%、0.90%和3.59%,变幅分别为4.50~19.40 nkat·g-1、2.93%~5.95%、0.49%~1.35%和1.92%~5.01%。采用最长距离法对参试的68个小麦品种籽粒的LOX活性、总戊聚糖、水溶性以及非水溶性戊聚糖含量进行聚类分析,结果聚为4类。
关键词:  小麦  脂肪氧化酶  戊聚糖  聚类
DOI:
基金项目:国家自然科学基金(31071404, 31371615), 教育部高校博士点基金(20103418110002), 安徽省高校自然科学研究项目(KJ2012Z105)和安徽省资助创新项目(11Z0101080)共同资助。
Determination and cluster analysis of lipoxygenase (LOX) activity and pentosan content in 68 wheat varieties
WANG Hui,CUI Wenli,ZHANG Conghe,GU Mingchao,WU Ping,ZHENG Wenyin,ZHANG Wenming,YAO Danian
(School of Agronomy, Anhui Agricultural University, Hefei 230036; Anhui Win-all Hi-tech Seed Co. LTD., Hefei 230088)
Abstract:
Lipoxygenase (LOX) and pentosan are the most important factors which affect nutritional quality and processing quality of common wheat. Sixty-eight wheat varieties (lines) were selected to determine LOX activity, and the contents of total pentosan (TP), water-soluble pentosan (WSP) and water-insoluble pentosan (WIP), and variances among the wheat varieties were analyzed. The results indicated that the difference of LOX activity, content of TP, WSP and WIP were highly significant among the varieties. The means of LOX activity, the contents of WSP, WIP and TP were 10.35 nkat·g-1, 4.49%, 0.90% and 3.59%, respectively. The range of them were 4.50-19.40 nkat·g-1, 2.93%-5.95%, 0.49%-1.35% and 1.92%-5.01%, correspondingly . Based on the longest distance (Euclidean distance) method, all the varieties could be clustered into four major groups.
Key words:  wheat  lipoxygenase  pentosan  cluster analysis

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