引用本文:[点击复制]
[点击复制]
【打印本页】 【在线阅读全文】【下载PDF全文】 查看/发表评论下载PDF阅读器关闭

←前一篇|后一篇→

过刊浏览    高级检索

本文已被:浏览 44次   下载 66 本文二维码信息
码上扫一扫!
家蚕肠液蛋白酶消化生理参数的研究
程佳,刘朝良,王在贵,张宏福,杨文静,韩薇,程昌粟,项菲菲
0
(安徽农业大学生命科学学院,合肥 230036;中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室,北京 100193)
摘要:
家蚕肠液蛋白酶对家蚕消化吸收蛋白质及其生长发育有着十分重要的作用。为探明影响家蚕肠道蛋白酶活力的因素,从家蚕肠道内容物中分离出蛋白酶酶液,在不同温度、pH及金属离子处理条件下,用福林-酚法测定酶活力变化情况,确定家蚕肠液蛋白酶的最适作用条件,同时研究了家蚕中肠蛋白酶活力随龄期增长的变化规律。结果表明,在35℃、pH10.0左右条件下蛋白酶活力最大,低浓度Mn2+、Fe2+、Cu2+对酶活有抑制作用,高浓度Ca2+对酶有抑制作用,Mg2+浓度对酶活物影响不明显,家蚕中肠蛋白酶活力随龄期增长而增强。
关键词:  家蚕  肠道内容物  蛋白酶活性  福林-酚法
DOI:
基金项目:动物营养学国家重点实验室开放基金项目( 2004DA125184F1104), 转基因专项子项目“猪肽YY(Petide YY)蛋白功能研究”和国家星火计划项目(2013GA710092)共同资助。
Study on the digestion physiology parameter of protease from intestine of silkworm
CHENG Jia,LIU Chaoliang,WANG Zaigui,ZHANG Hongfu,YANG Wenjing,HAN Wei,CHENG Changsu,XIANG Feifei
(School of Life Science, Anhui Agricultural University, Hefei 230036;State Key Laboratory of Animal Nutrition, Institute of Animal Science of CAAS, Beijing 100193)
Abstract:
Protease in silkworm gut takes an important role in the protein digesting process and development of silkworm. In order to prove the factors influencing the protease activity in silkworm gut, we isolated the protease enzyme from the contents of silkworm gut, and determined the changing process of protease activity under different temperatures, pH values and metal ion concentrations by Folin phenol method, so as to make sure the most suitable conditions for the protease in silkworm gut. At the same time, the change of protease activity in midgut of silkworm with the increase of age was researched. The results indicated that the maximum activity of protease were at 35℃, and pH 10.1. The low concentrations of Mn2+, Fe2+ and Cu2+ and the high concentration of Ca2+ inhibited enzyme activity, while the concentration of Mg2+ showed significant effect on the enzyme activity. The protease activity in midgut increased greatly with the growth of silkworm.
Key words:  silkworm  intestine  proteinase activity  Folin-phenol method

用微信扫一扫

用微信扫一扫