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患爆发性败血症斑点叉尾鮰病原的分离与鉴定
陈红莲,王永杰,陈宇,潘庭双,吴明林,凌俊
0
(安徽省农业科学院水产研究所,合肥 230031)
摘要:
从患爆发性败血症斑点叉尾鮰肝、脾和肾分离到大量形态和色泽一致的细菌。随机挑选5株细菌注射健康斑点叉尾鮰,感染鱼72 h内全部死亡,且患病症状与自然发病症状一致,确认分离株为斑点叉尾鮰败血症的致病菌。依据其菌落形态、生理生化特征,结合其16S rRNA和gyrB基因序列分析,鉴定5个分离株为蜡样芽孢杆菌。用20种抗微生物药敏纸片对分离株进行药敏实验,发现5株菌均对7种喹诺酮类药物、5种氨基糖苷类药物,麦迪霉素,克林霉素和新生霉素敏感,而对2种头孢菌素类药物和复方新诺明有耐药性。
关键词:  斑点叉尾鮰  败血症  蜡样芽孢杆菌  16S rRNA  gyrB
DOI:
投稿时间:2013-05-21
基金项目:安徽省农业科学院院长青年创新基金项目(12B0515), 安徽省农业成果转化项目(12040302002)和安徽省农业科学院引进人才科研启动资金共同资助。
Isolation, identification of pathogenic bacteria from Ictalurus punctatus with fulminate septicemia
CHEN Honglian,WANG Yongjie,CHEN Yu,PAN Tingshuang,WU Minglin,LING Jun
(Fisheries Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031)
Abstract:
Large colonies with similar morphology and color were obtained from liver, spleen and kidney of channel catfish, Ictalurus punctatus with fulminate septicemia. The experiment was carried out with healthy juvenile channel catfish by means of injection with live 5 isolates randomly selected. All individuals of the fish injected died within 72 h. The symptom was similar to those of the naturally infected fish. Therefore, the isolates were suggested to be the pathogenic bacterium strain of Ictalurus punctatus with fulminate septicemia. These isolates were further identified as Bacillus cereus by phenotypic characters, analysis of their 16S rRNA and gyrB sequences. Antimicrobial sensitivity patterns of five strains were evaluated against 20 antimicrobial drugs. The result showed that they were all sensitive to seven quinolones, five aminoglycosides, midecamycin, clindamycin, novobiocin, and resistant to two cephalosporins and compound sulfamethoxazole.
Key words:  channel catfish (Ictalurus punctatus)  septicemia   Bacillus cereus  16S rRNA  gyrB

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