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天台鹅耳枥的组织培养与快速繁殖
陈珍,陈模舜,孙骏威,蒋明
0
(台州学院生命科学学院,台州318000;中国计量学院生命科学学院,杭州310018)
摘要:
为了首次建立天台鹅耳枥的组织培养与植株再生体系,以其茎段、未萌发的新芽及萌发后的嫩芽、叶片等为外植体进行离体培养试验。结果表明,以新生的嫩芽为外植体,成功诱导不定芽的分化与增殖,最佳培养基配方为1/2 MS+6-BA 2.0 mg·L-1+NAA 0.2 mg·L-1+PVP 0.2%。壮苗培养基配方为1/2MS+6-BA 0.5 mg·L-1+NAA 0.05 mg·L-1;最佳生根培养基配方为1/4 MS+IBA 1.0 mg·L-1+NAA 0.1 mg·L-1
关键词:  天台鹅耳枥  组织培养  不定芽增殖  PVP
DOI:
基金项目:浙江省台州市科技计划项目(1202TG01, 071KY07)资助。
Tissue culture and rapid propagation of Carpinus tientaiensis
CHEN Zhen,CHEN Mo-shun,SUN Jun-wei,JIANG Ming
(College of Life Sciences, Taizhou University, Taizhou 318000;College of Life Sciences, China Jiliang University, Hangzhou 310018)
Abstract:
To establish a system of tissue culture and plantlet regeneration from Carpinus tientaiensis, stem segments, buds, or buds under germination and leaves were used as explants. The results showed that the optimum medium for induction and multiplication of adventitious shoots was 1/2 MS+6-BA 2.0 mg·L-1+NAA 0.2 mg·L-1+ PVP 0.2% when buds was used as explants. The medium for cultivating strong seedling was 1/2 MS+6-BA 0.5 mg·L-1+ NAA 0.05 mg·L-1, and the optimum medium for root regeneration was 1/4 MS+IBA 1.0 mg·L-1+NAA 0.1 mg·L-1.
Key words:  Carpinus tientaiensis  tissue culture  multiplication of adventitious shoots  PVP

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