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绵羊抑制素A基因外显子1多态性及其与高繁殖力关系的研究
姜庆林,储明星,陈国宏,方丽,狄冉,刘秋月
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(扬州大学动物科技学院,扬州 225009; 广东省家禽科学研究所,广州 510520;中国农业科学院北京畜牧兽医研究所,农业部畜禽遗传资源与种质创新重点实验室,北京 100193)
摘要:
采用PCR-SSCP技术检测抑制素A(inhibin A,INHA)基因外显子1在高繁殖力绵羊品种(小尾寒羊和湖羊)和低繁殖力绵羊品种(多赛特羊、特克塞尔羊和德国肉用美利奴羊)中的单核苷酸多态性,同时研究该基因对小尾寒羊高繁殖力的影响。结果表明,在所有5个绵羊品种中都存在2种基因型(AA和AB)。高繁殖力的小尾寒羊和湖羊等位基因B的频率分别为0.21和 0.47,低繁殖力的多赛特、特克塞尔和德国肉用美利奴3个绵羊品种B等位基因频率分别为0.07、0.10和0.02。测序表明AB型和AA型相比在INHA基因外显子1中第877位发生T→C的碱基突变。独立性检验表明高繁殖力绵羊品种与低繁殖力绵羊品种之间INHA基因型分布差异显著(P<0.05)。AB型小尾寒羊平均产羔数比AA型多0.57只(P<0.01)。
关键词:  绵羊  高繁殖力  抑制素A基因  PCR-SSCP
DOI:
基金项目:国家肉羊产业技术体系建设专项资金(CARS-39), 国家科技支撑计划(2006BAD13B08), 国家973计划(2006CB102105), 国家自然科学基金项目(30300248), 北京市自然科学基金项目(5042017)和中央级公益性科研院所基本科研业务费专项(ywf-rc-1)共同资助。
Polymorphism of exon 1 of INHA gene and its relationship with prolificacy in sheep
JIANG Qing-lin,CHU Ming-xing,CHEN Guo-hong,FANG Li,DI Ran,LIU Qiu-yue
(College of Animal Science and Technology, Yangzhou University, Yangzhou 225009; Poultry Science Institute of Guangdong Province, Guangzhou 510520;Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193)
Abstract:
Inhibin A (INHA) gene was studied as a candidate gene for the prolificacy of Small Tail Han sheep. Single nucleotide polymorphism of exon 1 of INHA gene was detected in both high fecundity breeds (Small Tail Han and Hu sheep) and low fecundity breeds (Dorset, Texel and German Mutton Merino sheep) by PCR-SSCP. Results indicated that there were two genotypes(AA and AB)in all five sheep breeds tested. Frequency of allele B was 0.21 and 0.47 in prolific Small Tail Han and Hu sheep, respectively. Frequency of allele B was 0.07,0.10 and 0.02 in Dorset, Texel and German Mutton Merino sheep, respectively. Sequencing revealed a T→C mutation at 877 bp of exon 1 of INHA gene in the AB genotype in comparison to the AA genotype. The INHA genotype distributions were significantly different (P<0.05) among high fecundity sheep breeds and low fecundity sheep breeds. The Small Tail Han ewes with heterozygous genotype AB had 0.57 (P<0.01) lambs more than those with genotype AA.
Key words:  sheep  prolificacy  INHA gene  PCR-SSCP

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