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茶树根原花青素提取工艺及检测方法的优化
蒋晓岚,孟菲,刘亚军,万根文,吴珂,夏涛,高丽萍
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(安徽农业大学教育部/农业部茶叶生物化学与生物技术重点开放实验室,合肥 230036;安徽农业大学生命科学学院,合肥 230036)
摘要:
茶树(Camellia sinensis (L.) O. Kuntze) 中含有丰富的多酚类化合物,统称为茶多酚,包括黄烷醇、黄酮、黄酮醇、花青素、原花青素和酚酸类等。对茶树根中原花青素的提取工艺进行优化,筛选出最佳的提取工艺条件:丙酮浓度80%、提取时间35 min、提取温度50℃、加酸量0.25%。对多酚的检测方法—香草醛法、对二甲氨基肉桂醛 (DMACA) 法进行了系统性的优化,结果表明香草醛法最优条件:0~25℃、反应时间15 min、硫酸体积分数为50%、香草醛浓度为10 g·L-1。对二甲氨基肉桂醛(DMACA)法最优条件:0℃、5 min内、盐酸浓度1.2 mol·L-1、DMACA浓度2 g·L-1
关键词:  茶树  茶树多酚  提取工艺优化  检测方法优化
DOI:
基金项目:国家自然科学基金(31170647, 31170282和31270730), 安徽省自然科学基金(11040606M73)和安徽省高校自然科学基金(KJ2012A110)共同资助。
Optimization of extraction technology and detection method on proanthocyanidins in tea root
JIANG Xiao-lan,MENG Fei,LIU Ya-jun,WAN Gen-wen,WU Ke,XIA Tao,GAO Li-ping
(Key Laboratory of Tea Biochemistry and Biotechnology, Ministry of Education, Ministry of Agriculture, Anhui Agricultural University, Hefei 230036;School of Life Science, Anhui Agricultural University, Hefei 230036)
Abstract:
There are abundant polyphenol compounds in tea, collectively referred to as tea polyphenols, including flavanol, flavonoids, flavonols, anthocyanosides, proanthocyanidins and phenolic acids, etc. In this experiment, we systematically optimized those parameters affecting proanthocyanidins extraction rate. The best extraction conditions were as follows: 80% acetone, extraction for 35 min at 50℃ with 0.25%acid additives. The determination conditions with Vanillin method and Dimethylaminocinnamaldhyde method were also systematically optimized, and the results showed that the optimal condition with Vanillin method was 0-25℃, reaction for 15 min, 50% H2SO4 and 10 g·L-1 vanillin; while the optimal condition with DMACA method was 0℃, reaction for 5 min, 1.2 mol·L-1 HCl, and 2 g·L-1 DMACA .
Key words:  Camellia sinensis  tea polyphenol  optimization of process parameter  optimization of detection method

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