引用本文:[点击复制]
[点击复制]
【打印本页】【下载PDF全文】 查看/发表评论下载PDF阅读器关闭

←前一篇|后一篇→

过刊浏览    高级检索

本文已被:浏览次   下载 本文二维码信息
码上扫一扫!
抗淀粉样蛋白寡聚体单链抗体融合蛋白 在真核细胞中表达的初步研究
周维维,汪维云,刘瑞田,于晓琳
0
(安徽农业大学生命科学学院,合肥 230036;中国科学院过程工程研究所,生化工程国家重点实验室,北京 100190)
摘要:
阿尔茨海默病(Alzheimer’s disease, AD)是一种由脑内淀粉样蛋白(A()聚集引起的,以老年人记忆和认知功能丧失为临床表征的神经退行性疾病。研究表明,A(寡聚体是AD发生发展的主要因素。应用自行筛选出的能够特异识别淀粉样蛋白寡聚体的单链抗体W20基因,构建了以绿色荧光蛋白为报告基因的真核表达载体pSNAV2.0-W20-EGFP,并应用流式细胞分选、蛋白免疫印迹以及荧光显微镜等技术对该单链抗体融合蛋白在真核细胞中的表达进行了初步研究,为针对AD的抗体基因治疗奠定了基础。
关键词:  淀粉样蛋白  单链抗体(scFv)  阿尔茨海默病
DOI:
基金项目:国家自然科学基金项目(81171014)资助。
Expression of single-chain variable fragment antibody specifically recognizing beta-amyloid oligomers in 293T cells
ZHOU Wei-wei,WANG Wei-yun,LIU Rui-tian,YU Xiao-lin
(School of Life Science, Anhui Agricultural University, Hefei 230036;National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, China Academy of Sciences, Beijing 100190)
Abstract:
Alzheimer’s disease is the most common form of neurodegenerative disease that leads to a progressive loss of memory and cognitive functions. Increasing evidence indicates that beta-amyloid oligomers are pivotal toxins in the pathogenesis of Alzheimer's disease. In this study, W20, a single-chain variable fragment antibody specifically recognizing beta-amyloid oligomers was cloned to pSNAV2.0-EGFP vector and its expression in 293T cells was examined by flow cytometry, western blot and fluorescence microscope. Such scFv antibody eukaryotic expression vectors may have valuable application in the further investigation of AD gene therapy in vivo.
Key words:  beta-amyloid  single-chain variable fragment antibody  Alzheimer’s disease

用微信扫一扫

用微信扫一扫