引用本文:[点击复制]
[点击复制]
【打印本页】【下载PDF全文】 查看/发表评论下载PDF阅读器关闭

←前一篇|后一篇→

过刊浏览    高级检索

本文已被:浏览次   下载 本文二维码信息
码上扫一扫!
725杨组培繁殖技术研究
汪玲,项艳
0
(宿州学院特色种植业苗种生产工程技术研究中心,宿州 234000; 安徽农业大学林学与园林学院,合肥 230036)
摘要:
以美洲黑杨725杨树(Populus deltoides cl.‘725’)叶片为材料,对其再生体系的建立及其分化和生根苗对潮霉素B的浓度筛选进行了研究。结果表明,叶片的最佳消毒体系为75%的酒精消毒时间8 s,0.1%的升汞消毒3 min,最佳诱导愈伤的培养基是MS+6-BA 0.2 mg·L-1+2,4-D 1.5 mg·L-1+蔗糖25 g·L-1+琼脂6 g·L-1;最佳诱导分化培养基为MS+6-BA 1.0 mg·L-1+NAA 0.3 mg·L-1+KT 0.3 mg·L-1+蔗糖25 g·L-1+琼脂6 g·L-1;适宜的继代增殖培养基为MS+6-BA 0.3 mg·L-1+ NAA 0.05 mg·L-1+蔗糖25 g·L-1+琼脂6 g·L-1;最佳生根培养基为1/2MS+NAA 0.01 mg·L-1+ IBA 0.7 mg·L-1+蔗糖20 g·L-1+琼脂6 g·L-1;对725杨叶片分化和不定芽生根进行了潮霉素B的敏感性试验,确定叶片分化的临界浓度为2.5 mg·L-1,生根的临界浓度为1.5 mg·L-1
关键词:  725杨树  组织培养  再生体系  潮霉素B  浓度筛选
DOI:
基金项目:安徽省自然科学基金(1308085MC36)和宿州学院特色种植业苗种生产工程技术研究中心开放课题(2013YKF10)共同资助。
Research on tissue culture and propagation of Populus deltoides cl.‘725’
WANG Ling,XIANG Yan
(Engineering Technical Research Center of Characteristic Planting and Seedling Production, Suzhou University, Suzhou 234000; School of Forestry and Landscape Architecture, Anhui Agricultural University, Hefei 230036)
Abstract:
In this study, the regeneration system was established from leave explants of Populus deltoides cl.‘725’. Meanwhile, the buds and rooting seedlings of Populus deltoides cl.‘725’ were screened using hygromycin B. The results indicated that the best disinfection time of leaves was using 75% of the alcohol for 8 s and 0.1% HgCl2 for 3 min; The best suitable medium of deltoides cl.‘725’ leaves for callus induction was MS+6-BA 0.2 mg·L-1+2,4-D 1.5 mg·L-1+sucrose 25 g·L-1+agar 6 g·L-1; appropriate induction differentiation medium was MS+6-BA 1.0 mg·L-1+NAA 0.3 mg·L-1+KT 0.3 mg·L-1+ sucrose 25 g·L-1+agar 6 g·L-1; the best multiplication media was MS+6-BA 0.3 mg·L-1+NAA 0.05 mg·L-1+sucrose 25 g·L-1+agar 6 g·L-1; the best medium for rooting was 1/2 MS+ NAA 0.01 mg·L-1+ IBA 0.7 mg·L-1+ sucrose 20 g·L-1+agar 6 g·L-1; the critical hygromycin B sensitive concentrations for inducing shoots and roots of the poplar were 2.5 mg·L-1 and 1.5 mg·L-1, respectively.
Key words:  Populus deltoides cl.‘725’  tissue culture  regeneration system  hygromycin B  screen concentration

用微信扫一扫

用微信扫一扫