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MyoG基因单核苷酸多态性分析
杨漫漫,刘洪瑜,王恒,王力生,章孝荣,刘旭光
0
(安徽农业大学动物科技学院,合肥 230036;安徽农业大学动物科技学院,合肥 230036; 安徽地方畜禽遗传资源保护与生物育种省级实验室,合肥 230036)
摘要:
以皖东牛和广丰牛共128头牛为研究对象,采用PCR-RFLP方法检测牛MyoG基因的多态性。结果表明,在牛MyoG基因的外显子466碱基处发生G>C突变,检测到AA和AB 2种基因型,该位点的突变未引起氨基酸序列的变化。在皖东牛群体中AA基因型频率为23.66%,AB基因型频率为76.34%;在广丰牛群体中AA基因型频率为17.24%,AB基因型频率为82.76%。皖东牛的多态信息含量(PIC)为0.3606,广丰牛为0.3702,在该位点均处于中度多态。卡方检验表明皖东牛群体处于哈代-温伯格不平衡状态(P<0. 05);广丰牛处于哈代-温伯格平衡状态(P>0. 05)。
关键词:    MyoG基因  多态性
DOI:
基金项目:安徽农业大学青年科学基金重点项目(2010zd05), 安徽省农业成果转化项目(12040302077), 安徽省奶牛产业体系和安徽省肉牛产业体系共同资助。
Single nucleotide polymorphism analysis of MyoG gene in cattle
YANG Man-man,LIU Hong-yu,WANG Heng,WANG Li-sheng,ZHANG Xiao-rong,LIU Xu-guang
(School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036;School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036; Anhui?Provincial?Key?Laboratory?of?Local?Livestock?and?Poultry?Genetic?Resources?Conservation?and?BioBreeding, Hefei 230036)
Abstract:
One hundred and twenty-eight cattles from Wandong and Guangfeng populations were used to detect the polymorphism of MyoG gene by PCR-RFLP method. The results show that a G>C mutation was occur in 466 base position of exon 1, and 2 types of genotypes (AA and AB) were found. The mutation had not caused the change of amino acid. Genotypic frequencies of AA and AB are 23.66% and 76.34% respectively in Wandong population, the genotypic frequency of AA is 17.24% and AB is 82.76% in Guangfeng population. The polymorphism information content (PIC) is 0.3606 in Wandong population and 0.3702 in Guangfeng population, which are all in average polymorphism situation. According to the χ2 test, there is not in Hardy-Weinberg equilibrium in Wandong population (P<0.05) and there is in Hardy-Weinberg equilibrium in Guangfeng population (P>0. 05).
Key words:  cattle  MyoG gene  polymorphism

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