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源自合肥地区表观健康鸡的沙门氏菌耐药性与血清型、 基因型相关性分析
王元兰,刘军军,邵颖,方艳红,魏建忠,王桂军,孙裴,李郁
0
(安徽农业大学动物科技学院,合肥 230036)
摘要:
为了解源自合肥地区表观健康鸡的沙门氏菌耐药性及其与血清型、基因型的相关性,在前期已鉴定血清型和ERIC基因型的基础上,采用K-B纸片法对21株沙门氏菌分离株进行21种抗生素药物敏感试验,利用PCR技术对耐药菌株进行32种耐药基因检测。结果显示,总耐药率为100%(21/21),耐2种以上抗生素的菌株占76.19%(16/21),多重耐药谱为青霉素-氨苄西林-阿莫西林-链霉素-复方新诺明,其中耐受β-内酰胺类抗生素的类别最多,耐药率最高达100%(21/21)。21株沙门氏菌均检出耐药基因,涉及blaPSEsul1strA、blaTEM、sul2、tetA、tetB、aph3-ⅡacatⅠ 9种,其中blaPSE基因的检出率最高,为85.7%(8/21)。可见源自合肥地区表观健康鸡的沙门氏菌对β-内酰胺类抗生素耐药性最强,与其携带blaPSE基因有关;耐药表型测定结果与耐药基因检测结果基本一致;耐药性与具有相同来源的同一血清型、基因型相关。
关键词:  表观健康鸡  沙门氏菌  耐药性  血清型  ERIC基因型
DOI:
投稿时间:2013-01-23
基金项目:国家科技支撑项目(2009BADB9B01), 安徽省高等学校省级自然科学研究项目(KJ2008B056)和安徽省家禽产业体系基金。
Analysis of antimicrobial resistance on Salmonella isolated from nonsymptomatic chickens in clinic in Hefei and its serotype and genotype
WANG Yuan-lan,LIU Jun-jun,SHAO Ying,FANG Yan-hong,WEI Jian-zhong,WANG Gui-jun,SUN Pei,LI Yu
(School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036)
Abstract:
In order to investigate the relationship between antimicrobial resistance and serotypes, genotypes of Salmonella isolates from apparently healthy chickens in Hefei, the Kirby-Bauer method was applied to determine the sensitivity of 21 strains susceptibility to 21 antibiotics, and PCR technique also was used to detect 32 kinds of drug-resistance genes, based on identified serotypes and ERIC-PCR gene-type. It was found that 100%(21/21)of the Salmonella isolates were resistant to all antibiotics by Drug Susceptibility Test; 76.19% (16/21) were resistant to at least two antimicrobials among the 21 isolates, and the dominating multidrug-resistant spectrum was Penicillin G-Ampicillin-Amoxicillin-Streptomycin-Trimethoprim-sulfamethoxazole. It was the most extensive in resistance to β-lactamase antibiotics by 100%(21/21). Meanwhile, the resistance genes could be detected from all these isolates by PCR, including blaPSE, sul1, strA, blaTEM, sul2, tetA, tetB, aph3-Ⅱa and catⅠ, and blaPSE gene was the most prevalent with the detected rate of 85.7%(8/21). It is concluded that the stronggest antimicrobial resistance to β-lactamases drugs on Salmonella isolated from apparently healthy chickens in Hefei due to the existence of blaPSE gene; antimicrobial phenotype is also related to the antimicrobial resistance genes; antimicrobial resistance is in relevant with the same sources of the same serotypes and genotypes.
Key words:  apparently healthy chickens  Salmonella  antimicrobial resistance  serotype  ERIC-PCR gene-type

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