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猪肠源性枯草芽孢杆菌耐逆性研究
周明,王欢,李泽阳
0
(安徽农业大学动物科技学院,合肥 230036)
摘要:
研究了猪肠源性枯草芽孢杆菌对逆性因子的耐受性。将猪肠源性枯草芽孢杆菌作如下试验处理,在pH为2.0、3.0和4.0的营养液中培养 3、6和9 h,在含0.1%、0.3%和0.5%胆盐营养液中培养4和8 h,在70、80、90和100℃的营养液中处理10和20 min,在Cu和Zn离子浓度分别为0、100、200、300、400和500 mg·L-1的营养液中培养24 h,测定细菌存活率;在常规营养液中和37℃条件下培养24和48 h,测定培养液中细菌分泌的蛋白酶和淀粉酶活性。试验结果显示,猪肠源性枯草芽孢杆菌对酸和胆盐有较强的耐受性,对70~90℃的高温也有不同程度的耐受力;微量元素铜离子对枯草芽孢杆菌的增殖有促进作用,但锌离子对其有抑制作用;枯草芽孢杆菌有较强的产蛋白酶能力,但产淀粉酶性能较弱。基于试验结果推断,猪肠源性枯草芽孢杆菌对酸、胆盐、高温和铜离子的耐受性较强。
关键词:  猪肠源性枯草芽孢杆菌  逆性因子  耐受性
DOI:
投稿时间:2012-12-03
基金项目:
Study on resistance to stress factors of Bacillus subtilis from swine intestinal juice
ZHOU Ming,WANG Huan,LI Ze-yang
(School of Animal Science and Technology , Anhui Agricultural University, Hefei 230036)
Abstract:
The resistance to stress factors of bacillus subtilis from swine intestinal juice was studied. This strain was respectively inoculated in nutrition broth at pH 2.0, 3.0 and 4.0 for 3, 6 and 9 h,in 0.1%,0.3% and 0.5% bile salt nutrition broth for 4 and 8 h,in nutrition broth at 70,80,90 and 100 ℃ water bath for 10 and 20 min,and in nutrition broth containing 0, 100, 200, 300, 400 and 500 mg·L-1 of Cu and Zn ions for 24 h.The survival rate of bacillus subtilis was measured.Protease and amylase activities were determined after the bacteria was cultured at normal broth for 24 and 48 h, The results showed that Bacillus subtilis from swine intestinal juice had strong resistance to acid and bile salt, 71.8%-91.5% of the bacteria survived at temperature of 70-90℃. Cu ions could promote propagation of bacillus subtilis, but Zn ions could inhibit propagation of bacillus subtilis. Bacillus subtilis was able to produce high activity of protease, but low amylase. It is conclud that this bacteria has relatively strong resistance to acid, bile salt, high temperature and Cu ions.
Key words:  bacillus subtilis from swine intestinal juice  stress factors  resistance

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