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毛果杨(Populus trichocarpa)中ZINC-INDUCED FACILITATOR1(ZIF1)同源基因的生物信息学分析
李春艳,李成浩
0
(东北林业大学林木遗传育种国家重点实验室)
摘要:
以拟南芥ZIF1基因序列为基础,利用生物信息学软件及网站,找出毛果杨中ZIF1基因同源序列,对其进行生物信息学分析,并与其他物种中ZIF1同源基因序列进行多重比对与进化分析,使用实时荧光定量PCR技术验证毛果杨中ZIF1同源基因在锌胁迫条件下,根和叶中该基因的表达情况。结果预测出4个符合ZIF1基因基本结构特征,且具有与该基因相同保守功能结构域的毛果杨ZIF1同源基因,这4个基因属于稳定蛋白,可能具有信号肽,明显疏水区和典型跨膜区,基因定位在质膜上,实时荧光定量PCR结果表明,锌过量存在时,这4个基因表达上调,且根中最为显著。以上分析为进一步研究林木中ZIF1同源基因奠定了理论和实践基础。
关键词:  ZIF1  生物信息学  毛果杨
DOI:
基金项目:
Bioinformatics analysis of ZIF1 homologous gene in Populus trichocarpa
LI Chun-yan,LI Cheng-hao
(State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University)
Abstract:
According to the defined ZIF1 gene in Arabidopsis thaliana,bioinformatics software and websites were used to find out ZIF1 homologous gene in Populus trichocarpa,and to analyze the genes. The results were used to compare with ZIF1 homologous gene sequences of other species.In zinc stress condition, the expression of ZIF1 homologous gene in the roots and leaves of Populus trichocrpa was checked with real-time PCR. 4 ZIF1 homologous genes in Populus trichocarpa were predicted, and their basic structural characteristics and conserved domains of the 4 genes were the same to ZIF1 gene in Arabidopsis thaliana.The coding proteins of the four genes were stable with signal peptides, sharp hydrophobic regions and typical transmembrane domain localized in the plasma membrane.The real-time PCR results showed the 4 genes were up-regulation in the condition of excessive zinc,and it was significant in the roots. This study may lay a foundation for the further research of ZIF1 gene in forest.
Key words:  ZIF1  bioinformatics  Populus trichocarpa

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