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麻姑山林场球孢白僵菌遗传多样性的SSR标记分析
蒲顺昌,刘玉军,陈名君
0
(亳州师范高等专科学校;安徽农业大学安徽省微生物防治重点实验室)
摘要:
利用SSR(简单序列重复, simple sequence repeat)分子标记对来自安徽省麻姑山马尾松林的102株球孢白僵菌进行遗传多样性和基因分型研究。依据时间分类亚种群的Nei基因多样性(H)为0.1987,Shannon信息指数(I)为0.2789,亚种群间的基因分化系数(Gst)为0.1745,基因流(Nm)为1.2150;不同寄主亚种群的H为0.1945,I为0.2871,亚种群间的Gst为0.1664,Nm为1.3000。研究表明,麻姑山马尾松林球孢白僵菌有一定的遗传多样性,亚种群间遗传变异较小,有18个菌株属于同一基因型,亚种群内表现出较高水平的遗传分化。9对微卫星引物将102株白僵菌分成31个微卫星基因型。在这31种微卫星基因型中,有5种为相对优势基因型。31种基因型株系在不同月份中存在一定的动态变化,并通过侵染不同寄主昆虫,完成在森林生态系中的延续。并且,这些相对优势基因型并不是在各个月份均匀分布,在大部分月份中,存在1~2种优势度高的基因型。研究揭示,SSR分子标记在研究白僵菌的群体遗传结构和基因分型展现出快速和菌株的特异性特征。
关键词:  微卫星  基因分型  虫生真菌  遗传多样性
DOI:
基金项目:安徽省教育厅项目(KJ2013B152)和亳州师范高等专科学校校级科研项目(BSKY201112)
Genetic diversity analysis of Beauveria bassiana from Magu Mountainsof Anhui Province based on SSR molecular marker
PU Shun-chang,LIU Yu-jun,CHEN Ming-jun
(Bozhou Teachers College;Anhui Province Key Laboratory of Microbial Control, Anhui Agricultural University)
Abstract:
In this study, multilocus microsatellite genotyping and genetic diversity of 102 Beauveria bassiana isolates from Magu Mountains of Anhui Province were investigated using SSR (simple sequence repeat) molecular markers. For the sub-populations divided by sampling time, their Nei’s gene diversity (He), Shannon’s diversity index (I), coefficient of gene differentiation (Gst), and gene flow Nm were 0.1987, 0.2789, 0.1745 and 1.2150, respectively. However, for the sub-populations grouped based on host orders,the He, I, Gst, and Nm were 0.1945, 0.2871, 0.1664 and 1.3000, respectively. The results indicated that there was a certain genetic diversity among B.bassiana isolates from different time and the same geographical origins. Sub-interpopulations showed a low level genetic variation, with 18 isolates belonging to the identical genotype. However, sub-intrapopulations showed a high level genetic variation, and thirty-one multilocus microsatellite genotypes were determined for the 102 B. bassiana isolates using 9 primer sets. Five of the 31 genotypes possessed a higher level of relative dominance. The anniversary genotypes dynamics revealed that the five multilocus microsatellite genotypes continued to theirs spread and epidemicity in the local Masson’s pine plantation ecosystem by infecting different host. However, these relative dominant genotypes were not evenly distributed among different months, but in most months, only one or two dominant genotypes existed. The study suggested that SSR markers can be used as rapid and strain-specific molecular markers for the population genetics, and genotyping of Beauveria.
Key words:  microsatellite  genotyping  entomopathogenic fungi  genetic diversity

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