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茶树可溶性糖含量的HPLC-ELSD检测 及其与茶树抗寒性的相关分析
葛菁,庞磊,李叶云,江昌俊
0
(安徽农业大学茶叶生物化学与生物技术教育部重点实验室)
摘要:
建立了茶树叶片中可溶性糖的高效液相色谱一蒸发光散射检测(HPLC-ELSD)的测定方法。以Agilent Zorbax carbohydrate 柱为分离柱,流动相为乙腈-水(75:25, V/V),流速1.0 mL?min-1,柱温30℃,上样量10 μL。蒸发光散射检测器漂移管温度85℃ ,雾化器温度36℃。通过该方法测定越冬期间皖茶91、乌牛早、平阳特早和龙井43叶片中果糖、葡萄糖和蔗糖的含量变化。结果表明,越冬期间3种可溶性糖含量的变化幅度一致:皖茶91>龙井43>平阳特早>乌牛早。在茶树叶片中的含量均随着气温的降低呈现增长趋势,且具有较好的规律性。蔗糖作为茶树叶片中可溶性糖的主要成分,用HPLC-ELSD法可以方便、快速、准确的检测出来。因此低温胁迫下通过HPLC-ELSD法测定茶树叶片中蔗糖含量的变化,可作为鉴定茶树品种抗寒性的一种新方法。
关键词:  茶树  高效液相色谱  可溶性糖  越冬期  抗寒性
DOI:
基金项目:国家“十二五”科技支撑计划课题(2011BAD01B01)
Determination of soluble sugar in tea plant by HPLC-ELSDand its relationship with freezing resistance
GE Jing,PANG Lei,LI Ye-yun,JIANG Chang-jun
(Key Laboratory of Tea Biochemistry and Biotechnology, Ministry of Education, Agricultural University)
Abstract:
A HPLC-ELSD method for quantification analysis of the soluble sugar in the leaves of tea plant(Camellia sinensis) was developed under the following chromatographic condition: column, Agilent Zorbax carbohydrate,4.6×250 mm,10 μm ; mobile phase, acetonitrile-water (75:25, V/V); flow rate, 1.0 mL/min; column temperature, 30℃; sampling volume, 10 μL.The temperature of drift tube was 85℃, and the nebulizer temperature was 36℃. The contents of fructose, glucose and sucrose in the leaves of four tea tree species during overwintering periods were determined with this method. The results show that the change ranges of three soluble sugars are all in the order of Wancha91> Longjing43> Pingyangtezao > Wuniuzao. The soluble sugar content in the tea leaves has a regular increasing trend with decline of temperature. As the main ingredient of the soluble sugar, sucrose could be detected rapidly, simply and accurately by HPLC-ELSD. It’s a new way to determine the freezing resistance of tea plant.
Key words:  Camellia sinensis  high performance liquid chromatography (HPLC)  soluble sugar  wintering period  freezing resistance

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