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小鼠原始生殖细胞迁移过程中H2A.Z的表达
时小艳,吴宝江,于建宁,马雪山,刘红林
0
(江苏食品职业技术学院医药与健康管理系;内蒙古赛科星繁育生物技术股份有限公司;江苏省农业科学院畜牧研究所;中国科学院动物研究所;南京农业大学动物科技学院)
摘要:
小鼠原始生殖细胞(PGCs)迁移、增殖、性别分化都受着基因组和DNA表观遗传修饰的调控,H2A.Z与转录激活有关,可能与表观遗传修饰存在联系,通过PGCs单细胞及组织石蜡切片的免疫荧光方法进行研究。结果表明,PGCs在迁移过程中,8.5 dpc时PGCs中不存在H2A.Z;迁移至生殖嵴时H2A.Z主要集中于细胞核,11.5 dpc整个PGCs细胞核和细胞质都存在;13.5 dpc雌性的卵母细胞中H2A.Z主要集中于细胞质,而精原细胞中H2A.Z偏向集中于细胞核。综合基因组和DNA表观遗传修饰分析,H2A.Z的表达与其有密不可分的联系。
关键词:  小鼠原始生殖细胞(PGCs)  单细胞  石蜡切片  H2A.Z  免疫荧光
DOI:
基金项目:
Mouse primordial germ cells (PGCs) expression of H2A.Z during migration
SHI Xiao-yan,WU Bao-jiang,YU Jian-ning,MA Xue-shan,LIU Hong-lin
(Department of Medical and Health Management, Jiangsu Food Science College;Inner Mongolia Saikexing Reproductive Biotechnology Co. Ltd;Jiangsu Academy of Agricultural Sciences, Institute of Animal Husbandry in Jiangsu Province;Chinese Academy of Sciences, Institute of Animal;College of Animal Science and Technology, Nanjing Agricultural University)
Abstract:
Migration, proliferation and sex differentiation of mouse primordial germ cells (PGCs) is affected by the regulation of the genome and DNA epigenetic modification. H2A.Z is related to the transcription activation, whether there is a link with the epigenetic modification has not been involved. In this paper, PGCs single cell and tissue paraffin sections of immunofluorescence methods were used, and the results showed that during PGCs migration, H2A.Z did not exist in 8.5 dpc PGCs; with PGCs migration to the genital ridge, H2A.Z was mainly concentrated in the nucleus; H2A.Z was full of nucleus and cytoplasm in 11.5 dpc PGCs; H2A.Z was mainly in the cytoplasm in 13.5 dpc female oocytes, and H2A.Z tended to concentrate in the nucleus in spermatogonia. Therefore, the expression of H2A.Z is relevant to genomics and DNA epigenetic modification.
Key words:  mouse primordial germ cells (PGCs)  single cell  paraffin section  H2A.Z  immunofluorescence

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