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MiR-143在山羊卵巢组织中的表达及功能分析
张晓东,凌英会,李运生,张运海,张子军,丁建平,章孝荣
0
(安徽地方畜禽遗传资源保护和生物育种省级实验室,安徽省羊繁育工程技术研究中心, 安徽农业大学动物科技学院)
摘要:
旨在分析miR-143在妊娠与非妊娠山羊卵巢组织中的表达情况,并探讨其在山羊卵巢功能发挥以及其他生物学过程中所起的作用。采用Solexa测序及荧光定量PCR技术在妊娠和非妊娠山羊卵巢组织中检测miR-143的表达水平,并对其进行靶基因预测和GO富集分析。Solexa测序在山羊卵巢中检测到miR-143的表达,共发现miR-143及其异构体1 074个,总拷贝数为109 523,平均拷贝数为102;定量结果显示miR-143在妊娠山羊卵巢中的表达量是非妊娠山羊的12倍且差异显著(P < 0.05);GO分析表明miR-143广泛参与机体生殖发育、细胞增值分化等多个生物学过程。miR-143在妊娠山羊卵巢中的表达水平显著高于非妊娠山羊,而且其可能在卵巢激素分泌与作用应答、妊娠维持等生殖生物学过程中发挥潜在作用。
关键词:  miR-143  Solexa测序  定量PCR  GO分析  山羊卵巢
DOI:
基金项目:国家高技术研究发展计划(863)项目(2011AA100307-4),安徽省技术创新工程试点省专项(11Z0101095)和安徽省科技计划项目(11010302108)
Expression and biological function of MiR-143 in goat ovaries
ZHANG Xiao-dong,LING Ying-hui,LI Yun-sheng,ZHANG Yun-hai,ZHANG Zi-jun,DING Jian-ping,ZHANG Xiao-rong
(Anhui Provincial Laboratory for Local Livestock & Poultry Genetic Resource Conservation and Bio-Breeding, Anhui Provincial Engineering Research Centre of Reproduction and Breeding in Sheep, School of Animal Science and Technology, Anhui Agricultural Unive)
Abstract:
With the purpose of determining biological role of miR-143 in goat ovaries and many other biological processes, the differential expression of miR-143 between pregnant and non-pregnant goat ovaries was studied by Illumina Solexa sequencing and q-PCR technology, followed by target gene prediction and GO analysis. Solexa sequencing indicated that a total of 1 074 miR-143 isomiRs were identified in goat ovaies with 109 523 total read counts, and average expression level was 102. Q-PCR analysis showed that expression level of miR-143 in pregnant goat ovaries was 12 times more than that in non-pregnant goat ovaries, and the difference was significant (P < 0.05). The GO analysis showed that miR-143 played important roles in animal reproduction, cell proliferation and differentiation. MiR-143 may play potential roles in animal reproductive biological processes such as ovarian hormone secretion and pregnancy maintenance.
Key words:  miR-143  Solexa sequencing  q-PCR  GO analysis  goat ovary

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