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水稻黄叶突变体叶绿体超微结构与突变基因定位
武立权,李娟,柯建,何清华,尤翠翠,王荣富
0
(安徽农业大学农学院)
摘要:
对黄叶突变体-黄玉B的叶绿体超微结构和遗传特性进行研究。结果表明,野生型和突变体在相同的叶龄期,叶片内叶绿体个数差异不显著(P<0.05),但野生型的叶绿体基质浓厚、基粒片层堆叠比较整齐、有序,突变体基质较淡、基粒片层堆叠比较松散。遗传分析表明,该突变体黄叶性状受1对隐性基因控制,命名为xl(t);用SSR分子标记将xl(t)定位在第11染色体RM5349和RM21之间,遗传距离分别为0.82 cM和2.34 cM。
关键词:  水稻  黄叶突变体  叶绿体超微结构  基因定位
DOI:
基金项目:Anhui Provincial Natural Science Foundation (1208085MC34); National Scientific and Technological Support Project (2011BAD16B06, 2012BAD04B09, 2012BAD07B02, 2012BAD14B13)
Gene mapping and chloroplast ultrastructure of a xantha rice mutant
WU Li-quan,LI Juan,KE Jian,HE Qing-hua,YOU Cui-cui,WANG Rong-fu
(School of Agronomy, Anhui Agricultural University)
Abstract:
The chloroplast ultrastructure and genetic characteristics of a xantha rice mutant were studied in this paper. The result showed that the chloroplast number in leaves of mutant was not significantly different from its wild parent (P<0.05). Compared to its wild type parent, chloroplasts of the mutant had thin stroma and disorderly stacked grana lamellae. Genetic analysis demonstrated that the xantha leaf mutation was controlled by a single recessive gene named as xl(t) and found to be located between RM5349 and RM21 on chromosome 11 with the genetic distance of 0.82 cM and 2.34 cM, respectively.
Key words:  Rice (Oryza sativa L.)  xantha mutant  chloroplast ultrastructure  gene mapping

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