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高效液相色谱法检测蜂蜜中氟氯苯氰菊酯的残留
吴虹,余林生,檀华蓉,阮祥春,陈杨,宗超,苏红晓
0
(安徽农业大学蜂业研究所,合肥 230036;安徽农业大学生物技术中心,合肥 230036;安徽农业大学动物科技学院,合肥 230036;安徽农业大学茶与食品科技学院,合肥 230036)
摘要:
建立了蜂蜜中氟氯苯氰菊酯残留的高效液相色谱检测的方法。样品经正已烷-二氯甲烷(4:6, V/V)提取,旋转蒸发进行浓缩,Oasis HLB固相萃取柱萃取净化,在267 nm波长下用紫外检测器进行检测。通过前处理条件的优化大大减少了基质的干扰,氟氯苯氰菊酯的最低检测限达到0.005 mg·kg-1,平均回收率在80.8%~96.8%之间,变异系数为0.6%~1.5%。该方法精密度和重现性良好,适用于蜂蜜中氟氯苯氰菊酯残留的检测。
关键词:  氟氯苯氰菊酯  高效液相色谱法  蜂蜜
DOI:
基金项目:现代农业产业技术体系建设专项资金(CARS-45-KXJ9)资助。
Determination of flumethrin residues in honey by HPLC
WU Hong,YU Lin-sheng,Tan Hua-rong,RUAN Xiang-chun,CHEN Yang,ZONG Chao,SU Hong-xiao
(Apiculture Research Institute, Anhui Agricultural University , Hefei 230036;Biotechnology Center, Anhui Agricultural University, Hefei 230036;School of Animial Science and Technology , Anhui Agricultural University , Hefei 230036;School of Tea & Food Science and Technology, Anhui Agricultural University, Hefei 230036)
Abstract:
A method for determination of flumethrin residues in honey by using HPLC (high performance liquid chromatography) has been developed and validated. The sample was extracted from honey by the mix extractions of n-hexane and dichloromethane (4:6,V/V), concentrated by rotary evaporator, purified by HLB SPE cartridge, and detected at a wavelength of 267 nm. The pre-treatment conditions were optimized in order to decrease the matrix interference. The detection limit for flumethrin was 0.005 mg·kg-1. The average recoveries for flumethrin were between 80.8% and 96.8%. Coefficients of variation ranged from 0.6% to 1.5%. The method has good repeatability and high sensitivity, and can be applied for the determination of flumethrin residues in the honey.
Key words:  flumethrin  HPLC  honey

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