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不同枫树叶片中酚酸类物质高效毛细管电泳检测方法的研究
唐燕平,刘桂华,沈虎琴,田春秋
0
(安徽农业大学林学与园林学院,合肥 230036;安徽农业大学茶与食品科技学院,合肥 230036)
摘要:
采用正交试验的方法,研究了缓冲液pH、分离电压、运行温度等电泳参数对枫树叶片中酚酸类物质提取检测的影响,建立了4种槭树科枫叶中绿原酸、原儿茶酸、没食子酸和咖啡酸4种酚酸类化合物检测分离的高效毛细管电泳(HPCE)方法。得出了最佳分离条件为:紫外检测波长214 nm,缓冲液为20 mmol·L-1的磷酸-硼砂,pH 8.0,电压为22 kV,温度25℃。在此条件下,4种酚酸类化合物在9 min 内完全分离,且各组分浓度与峰面积呈良好的线性关系,r2 为0.9748~0.9965。迁移时间的RSD≤0.84%,峰面积的RSD≤5.29%,加标回收率为80.38%~96.88%。4种枫树叶片中均含有绿原酸,其中蚜虫宿主三角枫中绿原酸含量最低。
关键词:  HPCE  同时分离  酚酸类  枫叶
DOI:
基金项目:安徽省自然科学研究项目(2006 KJ 207B)资助。
Determination of phenolic acids in different maple leaves by high-performance capillary electrophoresis
TANG Yan-ping,LIU Gui-hua,SHEN Hu-qin,TIAN Chun-qiu
(School of Forestry and Landscape Architecture, Auhui Agricultural University, Hefei 230036;School of Tea & Food Science and Technology, Anhui Agricultural University, Hefei 230036)
Abstract:
In this paper, the effects of main parameters such as running buffer pH value, running voltage and column temperature on the extraction and detection of phenolic compounds from aceraceae leaves were investigated by orthogonal tests. A method was developed for the simultaneous determination of phenolic compounds in aceraceae maple leaves, including chlorogenic acid, protocatechuic acid, gallic acid, caffeic acid by high performance capillary electrophoresis (HPCE). At the condition of 20 mmol·L-1 potassium dihydrogen phosphate-borax buffer solution (pH 8.0), separation voltage of 22 kV, temperature of 25°C, UV detection wavelength of 214 nm, the four phenolic acid compounds were rapidly separated in 9 min. The calibration curves showed good linearity and the correlation coefficients were 0.9748~0.9965. Migration time,RSD ≤ 0.84%, and peak area RSD ≤ 5.29%. The average recoveries were from 80.38% to 96.88%. Chlorogenic acid could be detected in the four kinds of maple leaves, and the content in buerger maple which is the host of aphid was the least.
Key words:  high performance capillary electrophoresis  simultaneous separation  phenolic acids

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