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长座虫草子实体的人工诱导研究
刘洋,潘丹丹,丁海燕,李春如,樊美珍
0
(安徽农业大学微生物防治重点实验室,合肥 230036)
摘要:
首次应用长座虫草的无性型—长座被毛孢对长座虫草子实体的生长条件进行了研究,并确立了最佳的培养工艺。结果表明,长座被毛孢液体摇瓶最佳培养时间为9 d,在营养液中添加10%的动物性蛋白(黄粉虫粉)有助于提高子实体产量,其所需营养液最佳pH为6.0,培养温度为25℃,初期菌丝培养时间为完全黑暗培养,待菌丝长满后给予1~200 lx连续光照,可获得更多的子实体。
关键词:  长座虫草  长座被毛孢  子实体  人工诱导  培养条件
DOI:
投稿时间:2012-03-13
基金项目:国家高技术研究发展计划资助项目(863计划)(No.2007AA021506)和教育部留学回国人员科研启动基金(2009年)共同资助。。
Artificial induction of fruiting body of Ophiocordyceps longissima
LIU Yang,PAN Dan-dan,DING Hai-yan,LI Chun-ru,FAN Mei-zhen
(Anhui Provincial Key Laboratory of Microbial Control, Anhui Agricultural University, Hefei 230036)
Abstract:
The fruiting bodies of Ophiocordyceps longissima were cultured successfully using the strain of Hirsutella longissima RCEF3891, the anamorph of O. longissima, and the effect of different growth factors on fruiting bodies formation was studied in this paper. The results showed that the optimal culture conditions for fruiting bodies of O. longissima were as follows: 9 d of the liquid fermentation period of H. longissima, with addition of 10% animal protein in the medium, pH 6.0 of the nutrient solution, cultivation at 25℃, mycelia culture stage under the condition of complete darkness and continuous light (1-200 lx) after covering with mycelia.
Key words:  Ophiocordyceps longissima  Hirsutella longissima  fruiting body  artificial induction  cultural conditions

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