| 摘要: |
| 根据猫α－干扰素基因核苷酸序列，设计并合成了1对引物，PCR扩增狸猫α－干扰素全基因，并克隆、测序。用DNAStar软件及在线分析方法对克隆的IFN-α基因核苷酸及其推导氨基酸序列进行分析，并与人和其他种动物的IFN-α基因进行同源性和进化分析。结果表明，获得了狸猫α－干扰素全基因序列，其大小为631 bp，编码194个氨基酸的多肽，推导氨基酸序列有1个潜在的N-糖基化位点和7个潜在的O-糖基化位点。IFN-α基因存在种属的差异性，亲缘关系越近，同源性越高。狸猫IFN-α基因的成功克隆为进一步研究猫IFN-α基因表达、生物学活性和应用奠定基础。 |
| 关键词: 狸猫 α－干扰素基因 基因克隆 生物信息学 |
| DOI：CNKI：34-1162/S.20111025.1031.032 |
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| 基金项目:国家“十五”食品安全重大攻关专项（2001BA804A30-11）资助。 |
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| Cloning and bioinformatics analysis of IFN-αgene of wild cat |
| ZHU Qian-lei,WANG Ya-dan,CUI Bao-an,CHEN Hong-ying,WEI Zhan-yong,WANG Zi-xin,DUAN Ting-yun |
| (College of Animal Husbandry and Veterinary, Henan Agricultural University, Zhengzhou 450002;Luohe Entry-Exit Inspection and Quarantine Bureau, Luohe 462000) |
| Abstract: |
| One pair of primer was designed and synthesized according to the feline interferon alpha gene (IFN-α) nucleic acid sequence. Wild cat IFN-α was amplified by PCR, then cloned and sequenced. The nucleotide sequence of the amplified IFN-α gene and the amino acid sequence deduced from the gene were then analyzed by the DNAstar software and on-line tools, and the similarity and evolution of IFN-α gene were analyzed among wild cat, human and other animals. The results showed the full length sequence of wild cat IFN-α gene was 631 bp, which includes one open-reading frame, encoding 194 amino acid residues, one potential N-glycosalation site and seven O-glcnac sites in deduced amino acid sequence. The results of comparative sequence analysis and phylogenetic tree analysis indicated there was difference of genus in IFN-α gene, the nearer their relationship, the higher the homology. This study paved the way for future study of biological function and application of feline IFN-α. |
| Key words: wild cat interferon-α gene cloning bioinformatics |
