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狸猫α-干扰素全基因的克隆及生物信息学分析
朱前磊,王亚丹,崔保安,陈红英,魏战勇,王子馨,段廷云
0
(河南农业大学牧医工程学院,郑州 450002;漯河出入境检验检疫局,漯河 462000)
摘要:
根据猫α-干扰素基因核苷酸序列,设计并合成了1对引物,PCR扩增狸猫α-干扰素全基因,并克隆、测序。用DNAStar软件及在线分析方法对克隆的IFN-α基因核苷酸及其推导氨基酸序列进行分析,并与人和其他种动物的IFN-α基因进行同源性和进化分析。结果表明,获得了狸猫α-干扰素全基因序列,其大小为631 bp,编码194个氨基酸的多肽,推导氨基酸序列有1个潜在的N-糖基化位点和7个潜在的O-糖基化位点。IFN-α基因存在种属的差异性,亲缘关系越近,同源性越高。狸猫IFN-α基因的成功克隆为进一步研究猫IFN-α基因表达、生物学活性和应用奠定基础。
关键词:  狸猫  α-干扰素基因  基因克隆  生物信息学
DOI:CNKI:34-1162/S.20111025.1031.032
基金项目:国家“十五”食品安全重大攻关专项(2001BA804A30-11)资助。
Cloning and bioinformatics analysis of IFN-αgene of wild cat
ZHU Qian-lei,WANG Ya-dan,CUI Bao-an,CHEN Hong-ying,WEI Zhan-yong,WANG Zi-xin,DUAN Ting-yun
(College of Animal Husbandry and Veterinary, Henan Agricultural University, Zhengzhou 450002;Luohe Entry-Exit Inspection and Quarantine Bureau, Luohe 462000)
Abstract:
One pair of primer was designed and synthesized according to the feline interferon alpha gene (IFN-α) nucleic acid sequence. Wild cat IFN-α was amplified by PCR, then cloned and sequenced. The nucleotide sequence of the amplified IFN-α gene and the amino acid sequence deduced from the gene were then analyzed by the DNAstar software and on-line tools, and the similarity and evolution of IFN-α gene were analyzed among wild cat, human and other animals. The results showed the full length sequence of wild cat IFN-α gene was 631 bp, which includes one open-reading frame, encoding 194 amino acid residues, one potential N-glycosalation site and seven O-glcnac sites in deduced amino acid sequence. The results of comparative sequence analysis and phylogenetic tree analysis indicated there was difference of genus in IFN-α gene, the nearer their relationship, the higher the homology. This study paved the way for future study of biological function and application of feline IFN-α.
Key words:  wild cat  interferon-α  gene cloning  bioinformatics

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