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甲基睾丸酮在罗非鱼体内的药代动力学
洪二利,郑光明,孟娣,吴仕辉,戴晓欣,马兵,马丽莎,宋怿
0
(上海海洋大学水产与生命科学学院,上海200090; 中国水产科学研究院珠江水产研究所,广州510380;中国水产科学研究院,北京100141)
摘要:
以20 mg·kg-1和2 mg·kg-1剂量对经检测不含甲基睾丸酮残留的罗非鱼单次口灌给药,用高效液相色谱法测定血浆及组织中甲基睾丸酮(MET)的浓度。用乙酸乙酯提取样品,Florisil小柱净化。以3p97软件分析药时数据,MET在两个浓度组均符合一级吸收二室开放模型,吸收半衰期分别为0.29和0.28 h;分布半衰期分别为0.65 和0.57 h,达峰时间分别为是0.78和0.60 h,消除半衰期分别为18.40和5.98 h。20 mg·kg-1组血液和肌肉中MET残留至144 h仍有检出,至216 h血液和肌肉样品MET均未检出;肝脏中的药物浓度高于血液和肌肉中的浓度,MET残留至24 h有检出,48 h的样品未检出。结果表明,口灌给药,MET在罗非鱼体内吸收、分布快,肝脏为主要吸收和代谢器官,与血液和肌肉相比消除速率要快,血液和肌肉中的MET消除速率相对较缓慢,因此开展MET监督检验除了常规的肌肉样品的检测外,还应增加血液样品的检测。
关键词:  甲基睾丸酮  罗非鱼  药代动力学  残留消除  高效液相色谱法
DOI:CNKI:34-1162/S.20111025.1026.008
基金项目:中国水产科学研究院院部中央级公益性科研院所基本科研业务费专项资金项目(2008C018)资助。
Pharmacokinetics and elimination of methyl testosterone in tilapia
HONG Er-li,ZHENG Guang-ming,MENG Di,WU Shi-hui,DAI Xiao-xin,MA Bing,MA Li-sha,SONG Yi
(Faculty of Fisheries and Life Science, Shanghai Ocean University, Shanghai 200090; Pearl River Fishery Research Institute, Chinese Academic of Fishery Science, Guangzhou 510380;Chinese Academic of Fishery Science, Beijing 100141)
Abstract:
We determined the concentration of MET (methyl testosterone) in the plasma and tissues of tilapias using HPLC (high performance liquid chromatography) method. The condition of liquid chromatography is: using a C18 column, with methanol/ water (70∶30) as mobile phase, at 254 nm UV detection wavelength and 30℃ column temperature. Samples were extracted with acitate acid, purified with florisil column. Pharmacokinetics parameters and residues of MET were investigated in tilapia, after orally administered at a dose of 20 mg·kg-1 or 2 mg·kg-1. The C-T curves of both groups are in accordance with two compartment open model with the first order absorption. The pharmacokinetic parameters of MET in two groups are as follows: the absorption half-lives are 0.29 h and 0.28 h, respectively; distribution half-lives are 0.65 h and 0.57 h, respectively; the peak time are 0.78 h and 0.60 h, respectively; the elimination half lives are 18.40 h and 5.98 h, repectively. In group of 20 mg·kg-1, MET still could be detected in blood and mussel at 144 h, and MET could not be detected at 216 h; the concentration in liver is higher than that in blood and mussel, and MET could be detected at 24 h, while it could not be detected at 48 h. The results indicate that after oral administration, the assimilation and distribution of MET mainly absorbed and metabolized by the liver, is fast, while elimination of MET is slow. Therefore, in the course of MET supervision and inspection, both muscle and blood sample should be detected.
Key words:  methyltestosterone  tilapia  pharmacokinetic  residual elimination  high performance liquid chromatography (HPLC)

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