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茶树转录组中SSR位点的信息分析
杨华,陈琪,韦朝领,史成颖,方从兵,宛晓春
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(安徽农业大学农业部茶及药用植物安全生产重点开放实验室,合肥 230036; 安徽农业大学理学院,合肥 230036;安徽农业大学农业部茶及药用植物安全生产重点开放实验室,合肥 230036; 安徽农业大学园艺学院,合肥 230036)
摘要:
利用茶树全转录组的高通量测序获得的127 094条Unigenes来发掘茶树转录组SSR功能性标记。在这些序列中共搜索出12 242个SSRs,分布于10 325条Unigenes中,出现频率为9.63%。茶树转录组SSRs的平均长度为16 bp,平均分布频率是1/3.68 kb。在茶树转录组的SSRs中,二核苷酸重复是主要的类型,占总SSRs的63.78%。茶树转录组SSRs共包含181种重复基元,二核苷酸重复基元CT/AG和TC/GA是优势重复基元类型,分别占总SSRs的23.84%和23.58%。同时对这些SSR的可用性进行了评价。
关键词:  茶树  转录组  SSR信息
DOI:CNKI:34-1162/S.20111031.1102.001
基金项目:“十二五”国家科技支撑计划(2011BAD01B01)和教育部、农业部重点实验室开放基金项目(ltbb201102042)共同资助。
Analysis on SSR information in Camellia sinensis transcriptome
YANG Hua,CHEN Qi,WEI Chao-ling,SHI Cheng-ying,FANG Cong-bing,WAN Xiao-chun
(Key Laboratory of Tea & Medicinal Plants and Product Safety, Ministry of Agriculture, Anhui Agricultural University, Hefei 230036; School of Science, Anhui Agricultural University, Hefei 230036;Key Laboratory of Tea & Medicinal Plants and Product Safety, Ministry of Agriculture, Anhui Agricultural University, Hefei 230036; School of Horticulture, Anhui Agricultural University, Hefei 230036)
Abstract:
A total of 127 094 unigenes derived from deep sequencing of camellia sinensis transcriptome were used for the development of functional SSR molecular markers. Overall, 12 242 SSRs distributed in 10 325 unigenes were detected, accounting for 9.63% of all the unigenes. The average length and distribution distance of the transcriptomic SSRs are about 16 bp and 3.68 kb, respectively. There are 181 kinds of repeat motifs existing in the tea plant transcriptome, and the dinucleotide repeats are the main types, accounting for 63.78% of all the SSRs. CT/AG and TC/GA are the most frequent motifs, accounting for 23.84% and 23.58% in all the SSR repeat motifs, respectively. The potential of the transcriptomic SSRs for further usage and research was assessed.
Key words:  tea plant  transcriptome  SSR information

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