| 摘要: |
| 采用盐酸和甲醇抽提法，提取了枯草芽孢杆菌XF-1（Bacillus subtilis XF-1）的抑菌物质。以该提取物处理根肿菌孢子2 d，有50%孢子活性被抑制；处理9 d后，孢子萌发相对抑制率始终保持在90%以上。提取物对三七根腐病菌、水稻纹枯病菌、禾谷镰刀菌、魔芋基腐菌、烟草赤星菌生长抑制率分别为71.97%、62.23%、60.96%、60.70%和57.92%。 |
| 关键词: 枯草芽孢杆菌XF-1 根肿菌 拮抗物质 抑菌作用 |
| DOI：CNKI：34-1162/S.20110829.1430.011 |
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| 基金项目:科技部国际科技合作项目（2009DFA32360）, 农业部公益性行业（农业）科研专项（201003029）, 云南省自然科学基金重点项目（2008CC024）和云南省科技强省专项（2009EB060）共同资助。 |
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| Inhibition effect of Bacillus subtilis XF-1 extract on 11 fungal pathogens |
| JIA Yuan,SUN Rui-kui,WU Yi-xin,MAO Zi-chao,SUN Hai-xia,HE Yue-qiu |
| (School of Plant Protection, Yunnan Agricultural University, Kunming 650201; School of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201) |
| Abstract: |
| The antagonistic substance, isolated from the culture of Bacillus subtilis XF-1 by a method of hydrochloric acid precipitation conbined with methanol extraction substance, could inhibit 50% resting spore activity of Plasmodiophora brassicae treated for 2 d and over 90% spore germination treated for 9 d. The inhibition rates were 71.97%, 62.23%, 60.96%, 60.70% and 57.92% of the plant pathogens, i.e. Fusarium solani, Rhizoctonia solani, F. graminearum, F. solani, and Alternaria alternate, respectively. |
| Key words: Bacillus subtilis XF-1 Plasmodiophora brassicae antagonistic substance inhibition effect |
