引用本文:[点击复制]
[点击复制]
【打印本页】【下载PDF全文】 查看/发表评论下载PDF阅读器关闭

←前一篇|后一篇→

过刊浏览    高级检索

本文已被:浏览次   下载 本文二维码信息
码上扫一扫!
苏云金芽孢杆菌晶体杀虫蛋白Cry1Ac基因 在虫生真菌球孢白僵菌中的表达
刘振邦,任小芳,宋文婧,朱虹,黄勃,李增智
0
(安徽农业大学微生物防治省重点实验室,合肥 230036)
摘要:
依据苏云金芽孢杆菌晶体杀虫蛋白Cry1Ac基因序列设计引物进行PCR扩增,纯化产物与载体pbarGPE1连接,构建真菌表达载体pBARGPE1-Cry1Ac,通过芽生孢子转化法转入野生型球孢白僵菌中,获得多拷贝转化子;毒力测定结果表明转化子对马尾松毛虫的LC25比出发株降低5.4倍,LD25减少7.1倍,LT25缩短2.2 d,毒力明显提高;喂食处理和虫体喷雾喂食结合处理与单纯虫体喷雾处理相比,累计致死率分别提高了55.6%和63.0%,致死中时分别缩短了4.5 d和5.3 d。结果表明,苏云金芽孢杆菌晶体杀虫蛋白Cry1Ac基因的表达显著提高了球孢白僵菌的毒力。这是Cry1Ac基因首次在虫生真菌中表达。
关键词:  Bt毒蛋白  Cry1Ac 基因  球孢白僵菌  表达
DOI:CNKI:34-1162/S.20110829.1431.015
投稿时间:2011-04-26
基金项目:国家“863”项目(2006AA10A212), 安徽省高校省级自然科学研究重点项目(TD200708)共同资助。
Expression of Cry1Ac gene in entomogenous fungus Beaveria bassiana
LIU Zhen-bang,REN Xiao-fang,SONG Wen-jing,ZHU Hong,HUANG Bo,LI Zeng-zhi
(Provincial Key Laboratory of Microbial Control, Anhui Agricultural University, Hefei 230036)
Abstract:
Bt delta-endotoxin Cry1Ac gene was cloned by PCR method and ligated with plasmid pbarGPE1 to construct a recombinant plasmid pbarGPE1-Cry1Ac. This recombinant plasmid pbarGPE1-Cry1Ac was then transferred into a wild-type strain of Beauveria bassiana by blastospore transform, resulting in a multicopy transformant. The results of bioassay against the Masson’s pine caterpillar, Dendrolimus punctatus,showed that LC25 of the transformant was reduced by 5.41 times, LD25 was decreased by 7.1 times, and LT25 shortened by 2.2 days, indicating a substantial virulence enhancement. Compared to spray treatment, the cumulative mortality of feeding treatment and feeding with spray treatment were increased by 55.6% and 63.0%, and LT50 shortened by 4.5 days and 5.3 days. These data indicated that the stomach toxicity of Bt delta-endotoxin gene enhanced the virulence of B. bassiana greatly. And this is the first expression of Cry1Ac gene in the insect fungi.
Key words:  Bt delta-endotoxin  Cry1Ac gene   Beauveria bassiana  expression

用微信扫一扫

用微信扫一扫