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奥奈达希瓦氏菌MR-1的Fe(Ⅲ)还原特性及其影响因素
陈洁,储茵,司友斌
0
(安徽农业大学资源与环境学院,合肥230036)
摘要:
研究奥奈达希瓦氏菌MR-1的Fe(Ⅲ)还原特性,并考察不同电子供体、不同形态Fe(Ⅲ)、溶解氧、pH等对奥奈达希瓦氏菌MR-1还原Fe(Ⅲ)的影响。结果表明,奥奈达希瓦氏菌MR-1对Fe(Ⅲ)有还原能力,以乙酸盐、乳酸盐和丙酮酸盐作为Fe(Ⅲ)还原的唯一电子供体,Fe(Ⅲ)还原率分别达到39.12%、50.89%和44.98%;以FeCl3、Fe(OH)3和柠檬酸铁等不同形态Fe(Ⅲ)为菌株MR-1的唯一电子受体,Fe(Ⅲ)还原率分别达到44.72%、51.54%和10.45%;溶解氧的存在可抑制Fe(Ⅲ)异化还原;菌株MR-1可在pH 5.0~9.0范围内进行Fe(Ⅲ)还原;两种不同的蛋白质变性剂SDS和OGP作用下的Fe(Ⅲ)还原结果表明,菌株MR-1的Fe(Ⅲ)还原功能蛋白主要位于细胞外膜;同时,投加一定量的单宁酸和焦性没食子酸可提高Fe(Ⅲ)还原率,螯合铁的还原率分别达到76.37%和68.73%。
关键词:  奥奈达希瓦氏菌MR-1  Fe(Ⅲ)还原  单宁酸  焦性没食子酸
DOI:CNKI:34-1162/S.20110624.1622.011
投稿时间:2011-03-30
基金项目:国家自然科学基金(40971182)资助。
Dissimilatory Fe(Ⅲ) reduction by Shewanclla oneidensis MR-1 and impact factors
CHEN Jie,CHU Yin,SI You-bin
(School of Resources and Environment, Anhui agricultural University, Hefei 230036)
Abstract:
Dissimilatory Fe(Ⅲ) reduction by Shewanclla oneidensis MR-1 was studied, and impact factors such as electronic donors, Fe(Ⅲ) forms, dissolved oxygen content and pH were investigated. The results showed that Fe(Ⅲ) could be reduced by Shewanclla oneidensis MR-1, and iron reduction rates were 39.12%, 50.89% and 44.98% using the acetate, lactate and pyruvate as the sole electronic donor of strain MR-1, respectively. The forms of Fe(Ⅲ) could influence their reduction, and iron reduction rates were 44.72%, 51.54% and 10.45% using ferric trichloride, ferric hydroxide and ferric citrate as the sole electronic receptor of strain MR-1, respectively. Dissolved oxygen inhibited the dissimilatory iron reduction. Strain MR-1 could reduce Fe(Ⅲ) in the range of pH 5.0 to 9.0. The effects of protein denaturants SDS and OGP suggested that Fe(Ⅲ) reduction function proteins of strain MR-1 were mainly localized to the soluble outer membrane fraction. Tannins acid and pyrogallic acid could enhance Fe(Ⅲ) reduction, and iron reduction rates were 76.37% and 68.73%, respectively.
Key words:  Shewanclla oneidensis MR-1  Fe(Ⅲ) reduction  tannins acid  pyrogallic acid

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