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绒毛狼尾草幼穗的愈伤组织诱导与植株再生
缪珊,范继红
0
(北京农业职业学院,北京 102442)
摘要:
以绒毛狼尾草幼嫩花穗为材料,研究了培养基种类和2,4-D对愈伤组织诱导以及6-BA对分化的影响。结果表明,MS较N6培养基适合绒毛狼尾草的组织培养。在附加3 mg·L-1 2,4-D的MS培养基中愈伤组织诱导率最高,达到100%。分化培养基以附加1.0 mg·L-1 6-BA的MS培养基为最佳,分化率为44.0%。在1/2MS培养基中附加0.5 mg·L-1 NAA和0.3 mg·L-1活性炭进行生根培养,根系生长健壮,生根率达100%。
关键词:  绒毛狼尾草  幼穗  组织培养  植株再生
DOI:CNKI: 34-1162/S.20110303.0952.014
投稿时间:2010-11-05
基金项目:北京农业职业学院院级科研基金项目(XY-QN-09-18)资助。
Callus induction and plant regeneration from young spike of Pennisetum setaceum
MIAO Shan,FAN Ji-hong
(Beijing Vocational College of Agriculture, Beijing 102442)
Abstract:
The effects of media types and concentration of 2,4-D on callus-induction with young spike of Pennisetum setaceum, and some concentrations of 6-BA on callus differentiation were studied. The result shows MS is the optimal medium on callus-induction. The callus induction rate reached its maximum of 100% at MS media with 3 mg·L-1 2,4-D, in the differentiation media with 1.0 mg·L-1 6-BA, the optimum differentiation rate was achieved at 44.0%, and in the 1/2 MS media plus 0.5 mg·L-1 NAA and 0.3 mg·L-1 activated charcoal, root generation rate reached 100%.
Key words:  Pennisetum setaceum , young spike, tissue culture, plant regeneration

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