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南抗杨叶片快速繁殖体系的建立
杨茹,项艳
0
(安徽农业大学林学与园林学院,合肥 230036)
摘要:
以南抗杨叶片为材料,对其快繁体系进行研究。选用9种分化培养基、4种增殖培养基和6种生根培养基,研究不同激素组合对快速繁殖体系建立的影响。结果表明,适宜的诱导分化培养基为MS + 6-BA 1.5 mg·L-1 + NAA 0.5 mg·L-1 + KT 0.1 mg·L-1;丛生芽增殖培养基为MS + 6-BA 1.2 mg·L-1 + NAA 0.3 mg·L-1 + KT 0.1 mg·L-1;最佳生根培养基为1/2MS + IBA 0.5 mg·L-1 + NAA 0.01 mg·L-1
关键词:  南抗杨  组织培养  快繁体系
DOI:CNKI: 34-1162/S.20110223.1639.015
投稿时间:2010-08-31
基金项目:国家科技支撑计划子课题(2009BADA6B06-1)和安徽省科技攻关重大专项(08010302073)共同资助。
Establishment of fast regeneration system from leaves of Populus deltoides cl. Nankang
YANG Ru,XIANG Yan
(School of Forestry and Landscape Architecture, Anhui Agricultural University, Hefei 230036)
Abstract:
This paper developed fast reproduction system from leaves of Populus deltoides cl.Nankang with 9 differentiation media,4 multiplication media and 6 rooting media. The effect of hormone concentration was also studied. The results indicated that the best differentiation media was MS + 6-BA 1.5 mg·L-1 + NAA 0.5 mg·L-1 + KT 0.1 mg·L-1; the best multiplication media was MS + 6-BA 1.2 mg·L-1 + NAA 0.3 mg·L-1 + KT 0.1 mg·L-1; the best rooting media was 1/2MS + IBA 0.5 mg·L-1 + NAA 0.01 mg·L-1.
Key words:  Populus deltoides cl. Nankang  tissue culture  fast regeneration system

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