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CSFV和PRRSV双重RT-PCR检测方法的建立及其应用
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摘要:
为建立一种能够同时快速诊断猪瘟(CSF)和猪繁殖与呼吸综合征(PRRS)2种传染病的方法,根据GenBank上已发表的猪瘟病毒(CSFV)、猪繁殖与呼吸综合征病毒(PRRSV)核苷酸序列,分别设计并合成2对能特异性扩增CSFV、PRRSV的引物,经过条件优化后,建立了检测CSFV、PRRSV的双重RT-PCR方法,扩增2种病毒的片段,大小分别为508bp和432bp.试验证明,该方法具有良好的特异性、敏感性、重复性和稳定性,简便、快速、高效,为CSFV与PRRSV的快速诊断及进一步的CSFV与PRRSV的流行病学研究提供了重要的技术支持.
关键词:  猪瘟病毒  猪繁殖与呼吸综合征病毒  双重RT-PCR
DOI:
基金项目:
Establishment and application of a double RT-PCR for classical swine fever virus and porcine reproductive and respiratory syndrome virus
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Abstract:
According to the nucleotide sequences of classical swine fever virus(CSFV)and porcine reproductive and respiratory syndrome virus(PRRSV)in GenBank,two pairs of primers were designed and synthesized.A double RT-PCR was developed based on these two pairs of primers which amplified the CSFV virus-specific segment with 508bp and the PRRSV virus-specific segment with 432bp in sizes after the conditions of PCR were optimized.The results indicate that the double RT-PCR reaction after optimization show a good sensi...
Key words:  classical swine fever virus  porcine reproductive and respiratory syndrome virus  double RT-PCR  

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