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甜瓜品种资源g-DNA提取及RAPD体系的优化
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摘要:
用改良CTAB法提取甜瓜基因组DNA,不同提取方法对DNA的质量和产率均有一定影响。用SPSS软件设计正交试验L9(33),对甜瓜基因组DNA的RAPD扩增体系进行优化,获得了RAPD-PCR反应组分的最佳组合及反应程序。对确定的反应体系进行试验,结果表明该体系稳定、可靠。
关键词:  甜瓜,基因组DNA,RAPD,正交表,扩增体系
DOI:
基金项目:
Extraction of genomic DNA and optimization of RAPD technique system in melon
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Abstract:
The changes of extraction method by CTAB have influence upon the quality and yield of the genomic DNA in melon.The orthogonal experiment L9(33) was made to investigate efficiency of the RAPD-PCR in different conditions,the optimal combination of ingredient in RAPD-PCR was established.The result of repeated experiment showed that the established system was stable and reliable.
Key words:  melon,genomic DNA,RAPD,orthogonal table,amplification system

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