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绞股蓝叶片DNA提取方法的比较研究
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摘要:
研究5种提取绞股蓝(Gynostemmna pentaphullum(Thumb)Makino)基因组DNA的方法,分别是简易法、高盐沉淀法、高盐低pH法、改良的SDS法和改良的CTAB法。提取结果经过琼脂糖凝胶电泳以及RAPD检测,5种方法均能提取出绞股蓝基因组DNA,提取的DNA数量多少依次是简易法、高盐沉淀法、高盐低pH法、改良SDS法和改良CTAB法,其中高盐低pH值法提取的DNA的RAPD图谱条带最亮、最清晰。从DNA的产量、质量以及实验耗时等方面综合比较,高盐低pH法为绞股蓝叶片DNA提取的最适方法。
关键词:  绞股蓝,基因组DNA,DNA提取
DOI:
基金项目:
Extraction methods of genomic DNA from Gynostemmna pentaphullum leaves
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Abstract:
Five extraction methods(basic method,high salt precipitation,improved SDS,low pH extraction medium with high salt and improved CTAB) of genomic DNA from Gynostemmna pentaphullum were studied.The DNA samples obtained by the above methods were tested by agarose gel electophoresis and RAPD.The results indicated that all methods could be used for Genomic DNA isolation form Gynostemmna pentaphullum.The yield of DNA extracted with basic method was the best,and then the high salt precipitation,improved SDS,low pH extraction medium with high salt and improved CTAB.The difference in yield,quality,time duration of the DNA samples were compared,and low pH extraction medium with high salt was considered to be fast and reliable technique among the five methods.
Key words:  Gynostemmna pentaphullum,genomic DNA,DNA extraction

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