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水稻组蛋白脱乙酰化酶SIR2的功能注释分析
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摘要:
依赖于NAD 的组蛋白脱乙酰化酶SIR2对酵母和哺乳动物细胞的存活、衰老、凋亡等生理活动的调节起着重要的作用.利用生物信息学方法对SIR2在水稻中的生物学功能进行研究.结果表明,SRT701和SRT702分别位于第4条和第12条染色体上;它们编码的蛋白均含有N-糖基化、蛋白激酶C磷酸化、酪蛋白激酶Ⅱ磷酸化和豆蔻酰化4个相同的位点;SRT701主要位于细胞核,而SRT702主要位于内质网,它们均有跨膜区,且为疏水性蛋白质,都无信号肽;多序列比对发现植物SIR2家族的SIR2结构域高度保守;电子表达谱分析发现SRT701主要表达于叶片和花序,SRT702表达于整个植物中.利用RT-PCR方法进行检测的结果表明,SRT701在水稻根、茎、叶中均有表达,而SRT702只在叶、茎中有表达,根中没有表达,说明利用生物信息学方法所得的结果只能作为初步预测.水稻SRT701和SRT702器官特异性的表达模式表明它们可能在组织或器官形成中起着重要作用.
关键词:  组蛋白脱乙酰化酶  生物信息学  SIR2  RT-PCR
DOI:
投稿时间:2006-03-24
基金项目:国家自然科学基金 , 广东省自然科学基金 , 华南植物园科研启动经费
Analysis of function notation of histone deacetylase SIR2 family in rice by bioinformatics strategy
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Abstract:
The silent information regulator protein(SIR2) and its homologs(collectively known as Sirtuins),NAD -dependent deacetylase enzymes,play an important role in physiological regulation,such as the survival,aging and apoptosis of cell.In order to investigate the function of SIR2 in rice,bioinformatics was used to analyze the SIR2 family.SRT701 and SRT702 were localized in the 4 and 12 chromosome of rice,respectively,and their coding proteins all have the N-glycosylation site,protein kinase C phosphorylation site,the Casein kinase II phosphorylation site,and the N-myristoylation site.SRT701 localizes in the nuclear,whereas SRT702 localizes in the endoplasmic reticulum.Both SRT701 and SRT702 all have the transmembrane regions,and they are hydroproteins without signal peptide.Plant SIR2 family members were aligned by Clustal W and their domains are highly conserved.The analyses of the electronic expression profile of SRT701 and SRT702 indicated that SRT701 was expressed in the leaf and whereas the panicle SRT702 was expressed in the whole plant.In comparison,RT-PCR analysis indicated that SRT701 was expressed in the root,stem and leaf while SRT702 was expressed in the stem and leaf,but not in the root.Our analysis indicated the results predicted by the bioinformatics can be predicted but must be confirmed by the experiment methods.The organic expression profile of SRT701 and SRT702 suggest that they may play an important role in the growth and development of rice.
Key words:  histone deacetylase  bioinformatics  SIR2 family  RT-PCR

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