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济宁青山羊MTNR1A基因外显子2的克隆与序列分析
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摘要:
对常年发情的山羊品种济宁青山羊和季节性发情的山羊品种辽宁绒山羊共20只母羊的褪黑激素受体1A(melatonin receptor 1A,MTNR1A)基因外显子2的824 bp扩增产物进行了克隆测序及序列比较分析.结果表明,济宁青山羊MTNR1A基因外显子2的核苷酸序列与已发表的山羊序列(GenBank登录号AF419334)完全相同.济宁青山羊与辽宁绒山羊MTNR1A基因外显子2的差异由11个核苷酸变化(A52G、T232C、T253C、A256G、T358G、T410A、A414T、C424T、A554G、T559C和C589A)组成,核苷酸同源性为98.7%.济宁青山羊与绵羊、母牛、猪、人、小鼠、挪威大鼠、西伯利亚仓鼠、鸡之间MTNR1A基因外显子2的核苷酸序列同源性为73.5%~98.4%,氨基酸序列同源性为79.2%~98.5%.
关键词:  山羊  繁殖季节性  褪黑激素受体1A基因  克隆  序列分析  济宁青山羊  基因外显子  克隆与序列分析  goats  Jining  gene  exon  sequence analysis  氨基酸  序列同源性  仓鼠  西伯利亚  大鼠  挪威  小鼠  母牛  绵羊  组成  变化  差异
DOI:
投稿时间:2005-07-18
基金项目:国家自然科学基金 , 北京市自然科学基金
Cloning and sequence analysis of exon 2 of MTNR1A gene in Jining grey goats
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Abstract:
The 824 bp fragment of exon 2 of melatonin receptor 1A(MTNR1A)gene was amplified successfully by PCR in both 10 does of nonseasonal estrous breed(Jining grey goats) and 10 does of seasonal estrous breed(Liaoning cashmere goats),and then cloned and sequenced.The results showed that the nucleotide sequence of exon 2 of MTNR1A gene in Jining grey goats was the same as that of goat published in GenBank(AF419334).The differences in exon 2 of MTNR1A gene between Jining grey goats and Liaoning cashmere goats consisted of eleven nucleotide changes(A52G,T232C,T253C,A256G,T358G,T410A,A414T,C424T,A554G,T559C and C589A),and the homology of nucleotide sequence between them was 98.7%.Homologies of nucleotide sequence and deduced amino acid sequence of exon 2 of MTNR1A gene between Jining grey goats and sheep,cow,pig,human,house mouse,Norway rat,Siberian hamster,chicken were 73.5% to 98.4% and 79.2% to 98.5%,respectively.
Key words:  goat  reproductive seasonality  melatonin receptor 1 A gene  cloning  sequence analysis

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