引用本文:[点击复制]
[点击复制]
【打印本页】【下载PDF全文】 查看/发表评论下载PDF阅读器关闭

←前一篇|后一篇→

过刊浏览    高级检索

本文已被:浏览次   下载 本文二维码信息
码上扫一扫!
携带绿色荧光蛋白的重组昆虫杆状病毒的构建
0
()
摘要:
将GFP cDNA片段克隆到昆虫杆状病毒的转移质粒pBacPAK8上,构建成携带绿色荧光蛋白的表达载体pBacPAK8-GFP;采用脂质体共转染法,将载体pBacPAK8-GFP DNA和亲本病毒Ac-BacPAK6 DNA共转染Sf21细胞,荧光均匀地分布于整个细胞.病毒经空斑分离法,成功分离病毒克隆,获得了重组绿色荧光蛋白的杆状病毒.
关键词:  昆虫  杆状病毒  绿色荧光蛋白  感染
DOI:
修订日期:2004-11-17
基金项目:江苏省教育厅科研项目 , 江苏大学校科研和教改项目
Construction of Recombinant Baculovirus Carrying Green Fluorescence Protein(GFP)
()
Abstract:
The gfp DNA fragments were amplified and inserted into baculovirus expression vector pBacPAK8. pBacPAK8-GFP DNA and viral Ac-BacPAK6 DNA were cotransfected into Sf21 cells with lipofectin. Observation under fluorescence microscope showed that the green fluorescence was spread in entire Sf21 cells,testifying expression of gfp to be easily detectable. The baculovirus plaques were picked up and the clones of baculovirus were isolated successfully.
Key words:  baculovirus,GFP,recombination,infection

用微信扫一扫

用微信扫一扫