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RT-PCR半定量法检测大蒜素对肉仔鸡肝脏HMG-CoAR、ACC和FASmRNA表达的影响
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摘要:
通过RT-PCR半定量法分析大蒜素对肉仔鸡肝脏中与脂类代谢有关的3种酶HMG-CoAR、ACC和FASmRNA表达的影响.结果显示,大蒜素抑制了肉仔鸡肝脏中HMG-CoAR、ACC和FASmRNA表达,下降率分别为80.8%、 74.2%和64.1%.
关键词:  肉仔鸡  HMG-CoAR  ACC  FAS
DOI:
修订日期:2003-05-10
基金项目:吉林省科技攻关项目
A Semi-quantitation RT-PCR Method to Examine the Influence of Garlicin on HMG-CoAR,ACC and FAS mRNA Expression in Chicken Livers
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Abstract:
The effects of garlicin on mRNA expression of HMG-CoAR, ACC and FAS,which were related to lipid metabolism in chicken livers were studied by a semi-quantitation RT-PCR method. The results showed that the mRNA expressions of HMG-CoAR, ACC and FAS in chicken livers were inhibited by garlicin,decreased by 80.8%,74.2%, and 64.1% respectively.
Key words:  chicken,HMG-CoAR,ACC,FAS

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