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山核桃基因组DNA提取及RAPD引物筛选
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摘要:
分别用SDS法、CTAB法、简易CTAB法及高盐低pH法提取山核桃干叶的基因组DNA并进行了检测比较.结果显示,SDS法更适合于山核桃基因组DNA的提取;对600条10碱基的随机引物进行了初筛和复筛,结果筛选出了20条扩增多态性强、稳定性好的引物,作为全部基因纽的扩增引物.
关键词:  山核桃  DNA提取  RAPD引物筛选
DOI:
修订日期:2004-02-27
基金项目:国家自然科学基金 , 浙江省科技厅资助项目 , 浙江省自然科学基金 , 浙江省杭州市科技局资助项目
Genomic DNA Extraction and RAPD Primer Screening of Carya cathayensis
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Abstract:
The methods of CTAB, rapid CTAB, SDS and low pH medium were used for genomic DNA isolation of Carya cathayensis.The results indicated that the method of SDS was the most suitable one for Carya cathayensis genomic DNA isolation. Twenty primers were screened from 600 kinds of 10 bp random primers, which could produce stable and polymorphic bands.
Key words:  Carya cathayensis,DNA extract,RAPD primer selection

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