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精噁唑禾草灵高效降解菌的分离与鉴定
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摘要:
取精噁唑禾草灵废水处理系统进水口处污泥进行驯化培养,分离到8株有效菌株,通过高效液相色谱分析这8株菌株降解精噁唑禾草灵的残存量,确定其中降解精噁唑禾草灵最好的一株菌株为本实验菌株,通过传统的微生物鉴定方法,将其鉴定为产碱菌属(Alcaligenes sp.),标记为Alcaligenes sp.H.分离菌株H可以以精噁唑禾草灵为唯一碳源和能源生长;在纯培养条件下,分离菌株H对较高浓度的精噁唑禾草灵(50 mg·mL-1和100 mg·mL-1)均能较好地降解.
关键词:  鉴定  Alcaligenes sp.H  精噁唑禾草灵  高效液相色谱
DOI:
修订日期:2004-05-11
基金项目:国家自然科学基金
Isolation and Identification of a Bacterial Strain for Degrading Fenoxaprop-p-ethyl
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Abstract:
Eight bacterial strains which could decompose fenoxaprop-p-ethyl were isolated from a batch culture with fenoxaprop-p-ethyl as carbon source and the sludge from waste water dealing system of Huaxing chemical engineering Co.Ltd. After determining the residual quantity of fenoxaprop-p-ethyl with HPLC , H was selected as dominant degradation bacterial strain for its best degradation ability to fenoxaprop-p-ethyl .Based on the morphological appearances and physiological characteristics,H was identified as Alcaligenes sp. and named as Alcaligenes sp.H., and Alcaligenes sp.H has a better biodegradation ability to high concentration fenoxaprop-p-ethyl with pure culture.
Key words:  identification,Alcaligenes sp.H,fenoxaprop-p-ethyl,HPLC

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