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新城疫病毒(NDV)HN基因真核表达重组质粒的构建
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摘要:
应用一对自行设计的引物,通过反转录-聚合酶链反应(RT-PCR)获得新城疫病毒(NDV)Lasota株的血凝素神经氨酸酶(HN)基因片段,其长度约为1.7kb,与已报道的NDV-HN基因片段大小一致,再将该HN基因片段定向插入真核表达质粒pcDNA3中,经酶切和测序鉴定,表明构建的重组质粒含有NDV-HN基因片段。
关键词:  新城疫病毒 HN基因 免疫 真核表达重组质粒 构建 NDV 禽病
DOI:
修订日期:2001-11-22
基金项目:安徽省科技攻关项目;01013003;
Construction of the Eukaryotic Expression Recombinant Plasmid Containing NDV-HN Gene
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Abstract:
A cDNA fragment of hemagglutinin neuraminidae (HN) was obtained from Neucastle disease virus (NDV) Lasota strain by reverse transcription (RT) PCR with a pair of designed primers.Its length was about 1 7 kb, which conformed to the NDV HN gene fragment's reported in the literature. The fragment was inserted into pcDNA3, an eukaryotic expression plasmid by a directional link. The constructed eukaryotic expression recombinant plasmid contained NDV HN gene was identified by an endonuclease and DNA sequencing.
Key words:  neucastle disease virus (NDV),HN gene,eukaryotic expression plasmid,construction

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