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甲壳素亲和层析分离纯化稻胚凝集素
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摘要:
稻胚凝集素(RGL)能与N-乙酰氨基葡萄糖(GlcNAc)或其聚合物专一性结合。作者利用RGL与GlcNAc的天然多聚物-甲壳素有亲和作用的特性,以甲壳素为亲和层析固相物,从稻胚中分离出一种分子量、糖结合专一性、等电点等与文献报告的RGL性质一致的凝集素。
关键词:  亲和层析 稻胚凝集素 甲壳素 分离 纯化
DOI:
修订日期:2000-12-08
基金项目:
Purification of RGL from Rice Germ by Affinity Chromatograph on a Chitin Column
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Abstract:
Rice germ lectin (RGL) could be combined with N acetyl D glycosamine (GLcNAc) or its polymer specially.The affinity isolation of RGL from rice germ by affinity chromatograph on a chitin column was carried out in this paper.The isolated lectin had similar molecular weight,biological specificity and isoelectric point compared with the reported RGL.
Key words:  RGL,affinity chromatograph,chitin

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