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产黄青霉几丁质合成酶基因的克隆及系统学分析
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摘要:
通过对产黄青霉基因组DNA进行PCR扩增得到几丁质合成酶基因的同源片段。经克隆及序列分析表明所得,片段为4种不同基因,分别命名为PcCHS1,PcCH-S2,PcCHS3及PcCHS4,经比较演绎氨基酸序列,PcCHS1属于I型几丁质合成酶,Pc-CHS2及PcCHS3为Ⅱ型,PcCHS4为Ⅲ型。为了进行产黄青霉的系统学研究,将几丁质合成酶基因保守区的演绎氨基酸片段进行同源性搜索及引导序列分析,结果表明青霉菌与子囊菌,尤其是曲霉属真菌具有紧密的演化关系。几丁质合成酶引导序列分析结果也支持了几丁质合成酶在丝状真菌系统学研究中的有效性。
关键词:  产黄青霉 几丁质合成酶 克隆 测序 系统发育
DOI:
基金项目:
Cloning and Phylogenetic Analysis of Chitin Synthase Coding Genes of Penicillium chrysogenum
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Abstract:
DNA fragments homologous to chitin synthase were amplified from the genomic DNA of Penicillium chrysognum by PCR. Cloning and sequencing of the PCR amplified fragments led to the identification of four different genes, designated PcCHS1, PcCHS2, PcCHS3, and PcCHS4. By comparison of the deduced amino acid sequences, PcCHS1 was identified as a gene for class I chitin synthase, PcCHS2 and PcCHS3 were for class II, and PcCHS4 was for class III. For the phylogenetic study of P. chrysogenum, the Blast P similarity search and the bootstrap analysis of the deduced amino acid sequences of the highly conserved region of chitin synthase genes were performed. The analysis of amino acid sequences implicated a close evolutionary relationship of Penicillium to ascomycetous fungi, especially to genus Aspergillus. The results from bootstrap analysis of the chitin synthase also supported the usefulness of the chitin synthase for phylogenetic study of filamentous fungi.
Key words:  Penicillium chrysogenum Chitin synthase Clone Sequencing Phylogentics

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