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韩国果园菌根真菌的遗传特性
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摘要:
运用现代分子技术对分离于韩国果园中的共生真菌进行了鉴定,经显微观察及PCR-RAPD分析可将收集菌株分为不同组群。不同植物、不同真菌的共生反应类型不同,所有采集的真菌分离株均能刺激果树根的生长,有时甚至能刺激兰属温带果树茎干的生长。5种真菌的18s rDNA序列经BLAST软件分析,证明它们分属于3个组,其中有2个种;葡萄丝粒菌及内生丝核菌可以确定,基于18 s rDNA树状分枝图与植物反应类型的不同相一致,但与PCR-RAPD多态性分析结果不同,韩国当地春兰分离株与严重的土传病害丝核菌及镰刀菌不同,这种不同可通过不同引物的PCR-RAPD技术进行鉴别。
关键词:  共生 丝核菌 土壤病害 根部疾病 果园菌根真菌
DOI:
基金项目:
Characterization of the Orchid Mycorrhizal Fungi Isolated from Roots of Korean Native Orchids
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Abstract:
The orchid symbiotic fungi were isolated and identified from the roots of Korean native orchids using modern molecular techniques. The selected isolates were segregated into several groups based on microscopic observations and PCR RAPD results, and symbiosis responses were different for the different fungi on the various plant species. All fungi isolates selected stimulated the root growth for all orchid species tested, and some affected the shoot growth of the Cymbidium temperate orchid.The five fungi 18s rDNA sequences were analyzed using BLAST. Based on the tested different characteristics, the fungal isolates were segregated into three groups.Two of these three symbiotic isolate groups were confirmed as Rhizoctonia repens (P01) and Rhizoctonia endophytica var. endophytica (P02).The patterns of the dendrograms made based on sequences of 18s rDNA were consistent with patterns of dendrograms obtained from the plant responses, but not consistent with the polymorphisms indicated by PCR RAPD. The fungal isolates collected from C. goeringii, a Korean native orchid, were different from plant pathogenic species of Rhizoctonia and Fusarium, well known as a soil borne disease. Also, the techniques to distinguish the symbiotic isolate from the pathogenic fungus was developed by using the PCR RAPD reacted with the primers employed.
Key words:  Orchid Symbiosis 18s rDNA PCR RAPD Rhizoctonia Mycorrhizae Soil borne disese Fusarium Root disease Plant response OMF

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