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猪心中提取和纯化辅酶Q10(联产CytC)
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摘要:
辅酶Q10和细胞以素C是促生物氧化酶类生化药物,本试验从猪心中提了和纯化辅酶Q10,并联产细胞以素C。猪心以酸性水液粗提,细胞色素C经人造沸石吸附,洗脱,盐析,三氯乙酸沉淀,弱酸性阳离子吸附,真空干燥得225mgCytC/kg猪心,纯度79%,提取后的猪心渣,用醇皂化法,经硅胶桂层板,无水乙醇结晶,重结晶,每千克猪心中撮辅酶Q1075mg,含量为95.3%。
关键词:  辅酶Q10 细胞色素C 化学提取 纯化 猪心 心脏
DOI:
基金项目:
Extraction and Purification of Coenzyme Q 10 and Cytochrome C from Pig Heart
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Abstract:
Coenzyme Q 10 and Cytochrome C are biochemical medicine of biooxidase.The artical has developed a method of extracting and purification of coenzyme Q 10 and cytochrome C from pig heart.Pig heart is ronghly treated in secondary acid liquid and 225 mg cytochrome C (purity 79%) from 1 kg pig heart is obtained through adsorption,eluting,salting out,precipitating,vacuum drying.75 mg coenzyme (Q 10 concentration 95 3%) is extracted from 1 kg pig heart residue through saponification,chromatography and crytallization.
Key words:  Coenzyme Q 10 Cytochrome C,

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