摘要: |
本文报道了应用聚丙烯酰胺凝胶垂直板电泳分析研究分布于稻田的三种不同体色泥鳅在心肌,骨骼肌,肝脏,眼,血清七种组织器官中LDH同工酶的表达特征。电泳图谱表明,这些组织分布和活力均具有明显的组织特异性;在脑组织LDH2活性最强,其它组织如血清,眼,卵巢,肝脏,心肌等组织中LDH3活力最强,此外,在肝脏中存在由Ldh-C编码的C带。 |
关键词: 泥鳅 LDH同功酶 稻田 |
DOI: |
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Characterization of the PAGE Patterns of LDH Isoenzymes of Migurnus anguillicaudatus Expressed by Three Kinds of Body Colours and Distributed over Rice Field |
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Abstract: |
The electrophoretic patterns of LDH isoenzymes in serum,eye,brain,o-vary,liver,skeletal muscle and cardiac muscle from adult M,anguillicaudatus expressed bythree dinds of body colours and distributed over rice field were studied by means of polyacry-lamide gel vertical electropheresis,The results indicated taht in these tissues there were twobands,namely LDH2 and LDH3,the activity of other LDH isoenzymes expressed low,evenno.The quantitative analysis exhibited a tissue specific expression in tissue distribution andenzyme-activity:the strongest activity of the LDH isoenzyme were LDH2 in brain,LDH3in other tissues,namely in serum,eye,ovary,liver and cardiac muscle.In addition,in liverthere was a C-band codied by Ldh-C. |
Key words: Migurnus anguillicaudatus LDH isoenzyme Tissue-specific, |