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泗棉3号体细胞胚发生和植株再生
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摘要:
本文探讨了我国优良品种泗棉3号组织培养胚胎发生和植株再生所需要的条件。子叶、胚轴与胚根在不同ZT浓度的培养基上均能诱导形成愈伤组织,其中以3.0mg/L和5.0mg/LZT最有利于愈伤组织的诱导。随着ZT浓度的增加,外植体分化根的能力减弱。
关键词:  棉花 泗棉3号 胚胎发生 植株再生
DOI:
基金项目:
Somatic Embryogenesis and Plant Regeneration of An Elite Cotton Variety, Simian No.3
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Abstract:
The condition for somatic embryogenesis and plant regeneration of an elite cotton variety ,simian No,3 in vitro culture was investigated.callus could be produced from various explants,and the suitable ZT concentration was 3.0 or 5.0 mg/L. The root differentiation ability ofexplants decreased with the ZT comcentration increased.Embryogenic callus produced somaticembryos of various development stage when transfered to MS medium after 20 days subculture.Mature embryos were transfered into germination medium and developed into plantlets. Thecallus induction ,embryogenesis and plant regeneration were influenced dominantly by ZTconcentration in the medium.
Key words:  Cotton,Simian No.3,Embryogenesis,Plant regeneration

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