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水稻悬浮培养细胞在固体和液体培养下的形态发生
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摘要:
藤板5号悬浮培养细胞经适当处理后,在固体分化培养基上形成大量的胚状体。观察到球形胚和胚柄结构,成熟胚状体为圆筒状,芽分生组织为叶原基包裹。胚状体的畸形发育和先熟萌发是导致胚状体苗转换效率低的主要原因。液体培养条件下通过逐步降低2,4-D浓度(一月内从1.0mg/1降至0.2m8/1)诱导了体细胞胚胎发生,且胚状体发生数量多,较为同步,细胞分裂也较为旺盛。
关键词:  细胞悬浮培养 水稻 体细胞胚胎
DOI:
基金项目:
Morphogenesis of Rice Cell Suspension Culturs in Solid and Liquid Culture Systems
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Abstract:
Rice cell suspension cultures were established from a Japonica variety,Fujisaka 5.Cell ag-gregates from the suspension cultures produced somatic embryoids including polyembyroids andmal- developed embryoids on solid regeneration after adequate mercuric chlorite treatment and ac-tivate charcoal preculture.The globular embryoids and the suspensor structure were observed.The mature somatic embryoid was wrapped by leaf primordium and was cylinder shaped.The so-matic embryiods gave poor plant transversion rate mainly due to mal-development and precociousgermination.Somatic embryogenesis could be induced homogeneously in liquid culture by gradu-ally reducing the concentration of 2 ,4-D in the medium from 1 mg/1 to 0.2 mg/1 in one month.Morphogensis could also be observed after longer suspension culture.
Key words:  Somatic embryogenesis,Cell suspension culture,Oryza sativa L.,

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