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“长富 2”苹果休眼条的催芽及其离体繁殖的研究
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摘要:
本文叙述了影响日本着色系富士“长富2”休眠条催芽及其离体繁殖和生根因子。经过冷冻处理的休眠条浸在BA(1mg/l)溶液中可产生最多的新枝条。首次接种的芽在有BA1mg/l和IBA0.1mg/l的LS培养基生长良好。MS比LS培养基更有利于微枝条的繁殖,其繁殖系数在一定范围内随培养时间增加而增加。生根以在含有NAA(0.25mg/l)和IBA(0.25mg/l)的混合培养基中为最好,有70%生根。(缩写词:LS,linsmain and Skoog;MS,murashige and Skoog;BA,6—苄基氨基腺嘌呤;NAA,萘乙酸;IBA,吲哚丁酸)。
关键词:  着色系富士苹果,休眠条,组织培养,催芽,微繁殖,微插条
DOI:
基金项目:
STUDIES ON THE SPROUTING OF THE DORMANT SHOOTS AND THEIR SHOOT PROLIFERATION AND ROOT INGTIATION OF THE JAPANESE APPLE ''''COLOURED FUJI No. 2'''' IN VITRO
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Abstract:
This paper describs the factors affecting the sprouting of the dormant sho-ots used for tissue culture, their shoot proliferation and root initiation of theapple"Coloured Fuji No. 2" in vitro. The best forcing solution for cold treateddormant shoots was the water with BA at I mg/1. The single node explantsestablished very well in LS medium with BA at I mg/1 and IBA at 0. 1mg/1.For microshoot proliferation,MS was better than LS medium. The proliferationcoefficient increased with the time of explants development. The NAA- containingmedium gave more roots than IBA- containing medium. LS medium withNAA at 0.25mg/1 and IBA at 0.25mg/1 gave the best root initiatin (70%rooting).
Key words:  Coloured Fuji No. 2,Dormant shoot,Tissue culture,Microproliferation,Microcutting

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