2007年第2期目录

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《安徽农业大学学报》征稿简则
  2007(2)
1.《安徽农业大学学报》为安徽农业大学主办的国内外公开发行的综合性学术刊物,面向国内外征稿,主要刊登农学、林学、畜牧、兽医、水产、土壤、茶叶、蚕桑、园艺、植保、农业工程、生物技术、环境科学以及相关基础学科的学术论文、研究报告等读者对象主要是农林科技工作者、高等学校师生以及有关部门的专业干部2.来稿要论点明确,数据可靠,论证严谨,文字简炼,标点准确按下列顺序行文:题名、作者姓名、$(地址、邮政编码)、中文摘要(100-300字符)、关键词、中国图书馆分类法分类号、正文、参考文献、英文题名、作者姓名的汉语拼音、$英文名称、…
  摘要[741]  PDF   HTML

虫生真菌与害虫微生物防治国际论坛大会报告文选

美国西部重要害虫摩门螽斯病原真菌的研究
  唐诺德·罗伯茨  2007(2):141-148
The Mormon cricket (MC),Anabrus simplex (Orthoptera:Tettigoniidae),has a long and negative history with agriculture in the western states of the USA where MC often migrates in large groups and causes significant damage to forage plants and cultivated crops.In this review, virulence to MC of isolates of the insect-pathogenic fungus Metarhizium anisopliae varieties acridum and anisopliae were compared in an effort to identify isolates with promise for use as MC biological control agents.All of the isolates tested induced 100% or nearly 100% mortality by six days post application of the fungal conidia.Searches for new Metarhizium isolates with high heat and UV-B tolerance included isolation fungi from field-caught MC and grasshopper after they died in the laboratory and culturing fungi from soil samples collected from numerous western USA sites.The survey was preceded by development of a dodine based selective medium that,at 0.002% active ingredient,permitted growth of M.anisopliae var.acridum,but inhibited most contaminating fungi.The M.anisopliae var.acridum isolates examined to date have much higher tolerance to heat and UV-B irradiation than M.anisopliae var.anisopliae isolates,and this may be critical to successful field applications.The variety acridum has not yet been found in the USA,so our search for such isolates continues.Several new M.anisopliae var.anisopliae and Beauveria spp.were found,and the Metarhizium isolates are being characterized as to stress tolerance and virulence to insects.Characterization includes comparisons of new and pre-existing Metarhizium isolates by amplified fragment-length polymorphism (AFLP) analysis.Finally, experiments on MC developmental biology were conducted to gather data needed to develop a degree day model and to establish laboratory colonies of MC.
  摘要[941]  PDF   HTML
在美国使用无纺布菌条防治光肩星天牛的前景
  安· 哈耶克  2007(2):149-156
An invasive long-horned beetle,Anoplophora glabripennis,was first reported in the northeastern and midwestern United States and eastern Canada between 1996 and 2004 and has been given the common name Asian longhorned beetle(ALB).This beetle has also been found in several countries in Europe.ALB is difficult to control because larvae are found within the wood of living trees and the long-lived adults often occur high in tree canopies.This species is native to China and Korea and,because it has been a major tree killer in China,government agencies in the U.S.and Canada are working to eradicate ALB from North America.Our laboratory has been developing a microbial control approach targeting ALB adults,based on the Japanese product Biolisa Kamikiri which is used to control cerambycids in orchards.Entomopathogenic fungi are grown within non-woven fiber bands(= fungal bands)and placed around tree trunks and branches where ALB adults become inoculated when walking across bands.We have conducted bioassays with Beauveria brongniartii,Beauveria bassiana and Metarhizium anisopliae against ALB larvae and adults to identify effective isolates and now focus our efforts on M.anisopliae F52(ARSEF 7711).Caged field trials conducted in China to compare fungal sprays with fungal bands(2000,2001)demonstrated decreased ALB longevity and fitness for both application methods but longer activity of fungi in cages treated with fungal bands compared with sprays.Uncaged field trials(2001,2002)yielded faster ALB adult mortality in fungal-treated plots and decreased fitness.Studies in New York City testing the longevity of activity of fungal bands in the field have documented that bands retain>1×107 conidia·cm-2(the threshold for activity of Biolisa Kamikiri)for over 3 months.In contrast,studies with unformulated conidia sprayed onto tree trunks in New York documented conidial survival of only a few days.Sublethal effects of exposure of adult female ALB to fungal bands have been investigated further in the laboratory.After either newly eclosed or reproductively active females are exposed to fungal bands,few viable larvae are produced before death of the females.When females are exposed to fungal bands and then caged with males,males become infected.
  摘要[762]  PDF   HTML
昆虫病原真菌在当前和未来的温室生态系统中的作用
  马克· 格特尔  2007(2):157-161
There are several biopesticides based on entomopathogenic fungi available in the market for use against insect pests in greenhouse ecosystems.Although most are compatible for use with predators and parasitoids in greenhouse ecosystems,much more research is needed to determine the intraguild interactions for each combination of host,pathogen,predator,and parasitoid.Our research has demonstrated that,although direct effects on the predators could be demonstrated in laboratory bioassays,different results were found under greenhouse conditions,indicating that results obtained in the laboratory may be a poor predictor of what occurs in the greenhouse.In both cases,additive effects were obtained under greenhouse conditions,demonstrating compatibility.In addition,there is increasing evidence that entomopathogenic fungi have significant potential for dual management of invertebrate pests and plant pathogens.Our studies demonstrated that 3 species of Lecanicillium had significant effects on both aphids and cucumber powdery mildew,Sphaerotheca fuliginea;that the fungus Paecilomyces fumosoroseus was compatible with a mirid predator,Disyphus hesperus,when used concurrently against greenhouse whitefly,Trialeurodes vaporariorum;and that Lecaniciullium longisporum was compatible with a predatory midge,Aphidoletes aphidimyza when used concurrently against green peach aphids.
  摘要[1420]  PDF   HTML
瓦螨微生物防治的不良效果
  罗莎林· 詹姆士  2007(2):162-166
We report six different field trials testing the efficacy of Metarhizium anisopliae,an entomopathogenic fungus,against varroa mites in honey bee hives.Varroa mites are parasitic on honey bees and cause serious damage to Apis mellifera colonies.Several control methods are available for varroa mites,none are very effective,so new,more effective methods are being sought.Varroa has previously been shown to be highly susceptible to M.anisopliae infections,and in our first two field trials,we found some efficacy from spore applications.However,in subsequent field trials,we were not able to obtain any varroa control,despite attempting several different application methods,two different strains of the fungus,and testing in different climates and during different phenological states of the honey bee colony.We conclude that microbial control of varroa using fungi is not likely to be effective unless some way is found to prolong the survival of the spores(or other infective units)in the hive environment.
  摘要[1028]  PDF   HTML
利用昆虫病原真菌防治贮粮害虫展望
  杰夫· 劳尔德  2007(2):167-173
Only two fungus species,Beauveria bassiana and Metarhizium anisopliae,have been given serious attention as possible microbial controls for stored-product pests.Their host ranges are broad,but their potency for the various target insects varies greatly,and some of the most important pests such as the red flour beetle are very tolerant of fungi.Accordingly,strategies are needed to improve the fungal performance.One such strategy is combination with other environmentally benign treatments such as controlled desiccant dusts and controlled atmospheres.Ironically the relatively dryness of stored-product environments favors fungal efficacy.Desiccation stress renders some insects more vulnerable to fungi.The longevity of fungus spores is also best under dry conditions.The prospects of fungi for control of stored-product pests can be improved by taking advantage of these phenomena and judicious selection of use venues and application strategies.
  摘要[732]  PDF   HTML
球孢白僵菌和苏云金杆菌拟步甲血清亚种联合使用防治马铃薯甲
  斯蒂芬· 瑞特  2007(2):174-184
This paper reviews the potential for using insect pathogens to control the Colorado potato beetle,Leptinotarsa decemlineata,and summarizes results from nearly 10 years of research by USDA-ARS-PPRU scientists aimed at developing methods and strategies for integrated use of Bacillus thuringiensis and Beauveria bassiana for biologically-based management of potato beetles in the northeastern USA.The described studies have resulted in 1)design of a tractor-mounted hydraulic spray system that improved targeting and consequent efficacy of microbial control agents,2)discovery of synergism between the bacterial pathogen Bacillus thuringiensis(Bt)and the fungal pathogen Beauveria bassiana(Bb),3)findings that substantial mortality of beetle larvae treated with B.bassiana during foliar spray programs did not occur until after they entered the soil to pupate,and 4)observations that spray applications made in the evening(at sunset)were significantly,but not substantially,more effective than applications made during the morning hours.These findings have led to development of a bio-based pest management system based on three spray applications made in the following order at 3-5-day intervals:Bt alone,Bt and Bb mixed,and Bb alone.This spray program,with the applications initiated at 50% egg hatch,is intended to control the actively feeding larval stages(providing protection from defoliation)and reduce survivorship to the adult stage(providing long-term control by reducing populations of overwintering adults).In the initial test of the integrated management system,beetle control and potato yields were statistically equivalent to those in plots in which the beetles were controlled with chemical insecticides.Demonstration of the effectiveness of this program will continue over the next 2-3 field seasons.
  摘要[866]  PDF   HTML
高大冷杉温室Strophosoma象甲的微生物防治
  夏洛蒂· 尼尔森,Jφrgen Eilenberg,Jφrgen Eilenberg  2007(2):185-194
In Denmark,the weevils Strophosoma melanogrammum and S.capitatum cause economic damage in Noble fir due to the adult stage feeding on the needles.No chemical treatments of these weevils are allowed in Denmark,so biological control is an attractive solution.We evaluated the potential for microbial control of larvae of Strophosoma spp.based on laboratory bioassays and field applications,taking effect on both target and non-target into consideration,as well as persistence of the applied fungus.In the laboratory Beauveria bassiana,Paecilomyces farinosus and Metarhizium anisopliae were able to infect and cause mycosis in Strophosoma larvae.Among the tested isolates the most virulent isolate was M.anisopliae BIPESCO 5,which resulted in 80 % mortality.In the field experiment M.anisopliae,isolate BIPESCO 5,was applied to the soil as a conidial suspension against larvae of Strophosoma spp.The effect of the fungus on the target population was monitored at a weekly basis by counts of emerging adult weevils during their activity periods.The population of Strophosoma spp.was reduced by up to 60% in treated plots compared to control plots.The non-target effects of M.anisopliae were studied by sampling insects and ticks from both treated and control plots.Seven days after treatment,two sampled insect orders(Hemiptera and Coleoptera)and ticks were found with prevalences of M.anisopliae above 50%,compared to no infection in the insects collected from control plots.Infections in coccinellids were found as long as 277 days after treatment.However,the effect on population level of non-target is still unexplored.The persistence of the fungus was documented by plating a soil suspension onto agar.We documented that conidia of M.anisopliae could persist in the greenery plantation for at least 418 days after application.
  摘要[812]  PDF   HTML
金龟子绿僵菌诱发UV-B抗性导致分生孢子产孢和孢子逆境耐受性的转化
  德罗奇欧· 兰格尔  2007(2):195-202
The conidial tolerance of Metarhizium anisopliae var.anisopliae isolate ARSEF 2575 to UV-B irradiation is greatly influenced by growth-environment alterations.In this review,we report high variability in conidial UV-B tolerance in response to altered culture conditions.Conidia produced on insect cadavers[Zophobas morio(Coleoptera)or Galleria mellonella(Lepidoptera)] had low tolerance to UV-B radiation;and conidia produced on potato dextrose agar supplemented with yeast extract(PDAY)had medium UV-B tolerance;whereas conidia produced on a minimal medium without any carbon source(MM),on MM with a non-preferred carbon source such as lactose(=MML),on PDAY plus 1 M NaCl or KCl,or PDBY with high alkalinity had the highest UV-B tolerances.All of the above conditions that induced high UV-B tolerance,however,also greatly reduced conidial production.Comparisons between stress tolerance and conidial production,particularly with conidia produced under osmotic and nutritive stress,point out that the benefits of producing very tolerant conidia have the enormous cost of low conidial production.Growth under visible light also greatly improved conidial UV-B tolerance,but light did not negatively influence conidial production.Therefore,culture on rich media under light is proposed as the most promising approach to producing conidia with improved UV-B tolerance for biological control of pest insects in agriculture.
  摘要[699]  PDF   HTML
中国虫生真菌应用50年简史
  李增智  2007(2):203-207
In China,there is a long history over 2300 years of entomogenous fungi used as medicines,but their use against insect pests did not start until mid 1950's.In 1970,the central government held a workshop to encourage the use of Beauveria bassiana against pine caterpillars,making the year a milestone in Chinese history of microbial pest control.Since then,quite a few fungi have been tried for control of over 60 forest and crop pests.In the past decades,low tech production has been being improved towards commercial production with higher techniques.Until 2006,5 products of fungal insecticides have been registered.Meanwhile,large scale application techniques have been being improved with molecular ecological approach towards a rational application strategy.
  摘要[889]  PDF   HTML
孕穗至灌浆期土壤渍水对冬小麦N、P、K素含量和积累量的影响
  魏凤珍,李金才,王成雨,屈会娟  2007(2):208-212
运用盆栽模拟土壤渍水逆境试验,研究孕穗期和灌浆期土壤渍水对不同小麦品种根系和地上部分N、P 、K素吸收和运转影响.结果表明,土壤渍水逆境对不同小麦品种N、P 、K素吸收的影响有异.孕穗期土壤渍水逆境显著影响根系对N、P素的吸收与运转 ,而对K素吸收与运转影响较小;灌浆期渍水逆境不仅影响根系对N、P、K素的吸收,同时也影响N、P素在地下与地上部器官的运转,但对K素在小麦体内的运转和分配影响较小.
  摘要[976]  PDF   HTML
不同基因型小麦及其近缘属黑麦的RAPD分析
  厉荣玉,林毅,蔡永萍,魏凤娟,张静  2007(2):213-217
采用随机扩增多态性DNA(RAPD)对来自我国不同生态区的21个普通小麦(Triticum aestirum L)品种以及3个小麦近缘属栽培黑麦(Secale cereale)品种的遗传差异进行分析.结果表明,黑麦与普通小麦间的分子标记多态性(66.20%)明显高于普通小麦之间的多态性标记(43.90%).冬春性不同的普通小麦品种间的多态性存在着较大的差异,冬性小麦品种间多态性(40.30%)明显高于春性小麦品种间RAPD多态性(14.66%).黑麦与普通小麦间的平均遗传距离(0.5086)是普通小麦品种间平均遗传距离(0.1378)的4倍.聚类分析表明,利用RAPD标记可以将黑麦和普通小麦以及冬春性普通小麦明显划分开来;来自同一选育单位或同一生态区的小麦品种大多都聚在一起.一些引物能对有些品种进行特异性扩增.因此,利用RAPD标记可以进行小麦品种指纹分析,确定基因型之间遗传差异,进一步划分小麦杂种优势群.
  摘要[886]  PDF   HTML
转bar基因小麦的回交转育研究
  郭宁,张玉江,江昌俊  2007(2):218-221
采用回交法,将抗除草剂转基因小麦bar基因导入皖麦48和扬麦158中.经苗期抗性鉴定及GS酶活性、叶绿素含量、PAT酶活性测定,结果证实了抗除草剂bar基因已经整合到小麦基因组中且能够正常表达.回交后代与对照在主要农艺性状上无明显差异,表明通过回交将bar基因转育到其它品种,是获得新的抗除草剂小麦品种的一条简便有效的途径.
  摘要[888]  PDF   HTML
不同海拔高度对毛竹主要物理力学性质的影响
  汪佑宏,田根林,刘杏娥,王传贵,柯曙华  2007(2):222-225
就不同海拔高度对毛竹主要物理力学性质影响的研究,为竹材资源合理开发、高效科学加工利用有着十分重要的意义.结果显示:(1)气干密度、基本密度,都随毛竹自身轴向高度及海拔高度增加而增加.山脚与山顶的毛竹基本密度分别为0.699、0.715 g·cm-3;气干密度分别为0.798、0.827 g·cm-3,在0.05水平上经T-检验差异显著.(2)山脚与山顶毛竹的抗弯弹性模量、顺纹抗压强度、抗弯强度分别为12 368.28、11 934.07,55.858、59.840和183.203、196.293 MPa,海拔高度对顺纹抗压强度、抗弯强度影响,在0.05水平上经T-检验差异显著.
  摘要[873]  PDF   HTML
栽植密度对小黑杨树木生长因子和木材物理力学性质的影响
  刘杏娥,汪佑宏,徐斌,张群  2007(2):226-231
以山西朔州小黑杨(Populus×xiaohei T.S.Hvang et Liang)人工林为试验材料,研究了3种栽植密度(1000、500、250株·hm-2)对小黑杨树木生长、木材生长量和主要物理力学性质的影响.结果表明,栽植密度对树木冠幅、冠长、胸径、湿心材面积、边材面积和木材基本密度、顺纹抗压强度和MOE的影响达到极显著水平;对湿心材比例、边材比例和MOR的影响达到显著水平;对基本密度的径向变异有一定程度的影响,但不改变其变异的一般趋势,即从髓心向外,先下降后呈上升的变化趋势.
  摘要[893]  PDF   HTML
晚籽银桂胚和胚乳发育的研究
  杨秀莲,向其柏  2007(2):232-234
用常规石蜡切片技术研究了晚籽银桂的胚和胚乳的发育过程.结果表明,晚籽银桂的胚胎发生类型为柳叶菜型.卵细胞受精后,合子经过一段时间休眠,于10月下旬进行分裂,经棒形胚、球形胚、心形胚、鱼雷胚,至果实成熟时发育为子叶胚.心形胚时期,胚柄最为发达.晚籽银桂的胚乳发育类型为细胞型.初生胚乳核先于合子分裂,胚乳细胞分裂较快,胚周围的胚乳细胞有降解现象.
  摘要[842]  PDF   HTML
湿地松人工林经验收获表的编制
  谢华,黄庆丰,周根土,徐向宇  2007(2):235-238
应用99块湿地松人工林标准地调查资料,以林分年龄、立地指数为自变量,利用Richards模型拟合各林分因子(直径、树高、蓄积等)生产过程;采用非线性麦夸特迭代求解法,确定各林分因子Richards模型参数,并进行模型检验和误差分析,在此基础上依据各林分因子生长模型编制16指数级湿地松人工林经验收获表.
  摘要[834]  PDF   HTML
不同年龄黄山松蒸腾速率及水分利用效率的比较研究
  武金翠,吴泽民,洪淑媛,石雷  2007(2):239-243
采用Li-6400便携式光合分析仪,研究不同年龄阶段的黄山松在不同季节的蒸腾及水分生理生态的变化.结果表明,3个年龄段黄山松1年生针叶的时蒸腾强度具有明显的日变化,均呈明显的双峰曲线,但是峰值出现时间不同;幼龄阶段的时蒸腾速率与时气孔导度的日变化趋势的基本相似,而中龄和中幼龄黄山松,其时均蒸腾速率与气孔导度的变化趋势具有明显的差异,说明幼龄黄山松受气孔的限制较明显;蒸腾速率日均值的季节变化3个年龄段黄山松均为夏季的蒸腾速率较高;各年龄段黄山松的水分利用效率变化曲线均为双峰型,年平均水分利用效率的排序为中幼龄(5.062)>幼龄(3.383)>中龄(3.011),中幼龄黄山松具有较高水分利用效率表明其能更好地适应干旱环境;通过季节均值比较,发现夏季黄山松具有较高的水分利用效率,是其能生长在土壤浅薄甚至岩缝中的一个主要原因之一.
  摘要[802]  PDF   HTML
栗茶间作模式对茶树光合特性的影响
  章铁,刘秀清  2007(2):244-247
对栗茶间作模式和单作模式下茶树叶片的光合生理生态特征分析表明,栗茶间作模式下净光合速率(Pn)日变化为单峰曲线,单作模式下净光合速率(Pn)日变化为双峰曲线;栗茶间作模式下茶树净光合速率(Pn)与胞间CO2浓度(Ci)和光合有效辐射(PAR)和大气温度(Ta)和叶面温度(Tl)呈显著相关,单作模式下茶树净光合速率(Pn)与胞间CO2浓度(Ci)和蒸腾速率(Tr)呈显著相关;栗茶间作模式和单作模式的回归方程分别为Pn=12.8713-0.0267Ci,Pn=23.1111 22.7827Cond-0.0770Ci.
  摘要[804]  PDF   HTML
大白菜叶片总RNA提取方法的研究
  甘德芳,王小军  2007(2):248-250
利用不同浓度的水杨酸溶液处理苗龄为7 d的大白菜植株,在处理12 h后提取粗酶液并测定酶活.分别采用Trizol试剂法、改良SDS法、异硫氰酸胍法等方法对酶活力最高试验组的叶片提取RNA进行RT-PCR反应.结果表明,0.6 mmol·L-1水杨酸浓度诱导试验组效果最佳,几丁质酶的活力达到最大值;改良SDS法提取的大白菜叶片总RNA完整性好、纯度高, 提取的RNA可用于RT-PCR及RACE等实验操作.
  摘要[801]  PDF   HTML
猕猴桃品种"皖翠"芽变位点的SCAR标记研究
  贾兵,朱立武,余兴,叶振风  2007(2):251-255
对 "海沃德"与"皖翠"RAPD扩增的2条分子量为740 bp、649 bp的特异带,进行了分离、纯化、克隆与测序,根据测序结果设计出4条20 bp的特异引物,进行SCAR-PCR扩增.SCAR-PCR扩增结果显示,分子量为649 bp的特异带在"皖翠"与"海沃德"中都扩增出了条带,说明"皖翠"这一RAPD扩增特异带并不存在;"皖翠"扩增出现了分子量为740 bp的特异带,而"海沃德"未出现此带,证明"海沃德"与"皖翠"存在此变异位点,成功地将"皖翠"变异的RAPD标记转化为SCAR标记,此标记可应用于"皖翠"品种的快速鉴定.
  摘要[747]  PDF   HTML
钾营养对中华猕猴桃叶片光合作用及叶绿素荧光的影响
  孙骞,杨军,张绍阳,张凤琪,丁士林  2007(2):256-261
对中华猕猴桃"81-5"品种采用4种钾水平处理.结果表明,NPK2处理可以增加猕猴桃叶绿素含量,显著提高光合速率、气孔导度和水分利用效率,降低叶绿素a/b值、叶片蒸腾速率和胞间CO2浓度.NPK2和NPK3处理可使一天中光合速率提前到达峰值,有效延缓植株的"午休".NPK1和NPK2处理能提高猕猴桃叶片的Fv/Fm和Fv/Fo,激活叶片电子传递活性和PSⅡ潜在活性、提高PSⅡ原初光能转化效率.缺钾和低钾会使叶片吸收的过剩光能通过光化学反应途径和非辐射能量途径耗散的能力降低.
  摘要[744]  PDF   HTML
连续施用猪粪菜地土壤基质化研究
  黄治平,徐斌,涂德浴  2007(2):262-264
采集连续施用0~4年猪粪的无公害蔬菜基地的温室土壤,研究连续施用规模化猪场猪粪的菜地土壤主要理化指标(pH、有机质、全氮和全磷、有效磷等)变化规律.并对测定数据进行了Fisher小样本非参数随机化显著水平检验.结果表明,连续施用猪粪,土壤pH值降低,有机质、全氮、全磷和有效磷增加.
  摘要[1010]  PDF   HTML
春季马唐-秋季冬萝卜红壤和黄壤中尿素氨挥发损失状况的研究
  崔键,周静,马友华,王国强,关静  2007(2):265-269
通过在耕作红壤和黄壤上施用尿素轮作马唐和冬萝卜,研究了春秋两季两种土壤尿素氨挥发损失的状况.结果表明,红壤和黄壤尿素氨挥发通量的趋势具有一致性,其最大值分别均出现在春季基肥的第7~8 d,秋季基肥的第7~8 d,模拟降雨追肥的第1~2 d.同种施尿素氮水平和养分管理下,氨挥发春季高于秋季,黄壤高于红壤.其中春季氨挥发红壤和黄壤分别为0.67和7.84 kg·hm-2,占施用氮素0.42%和4.90%;秋季氨挥发红壤和黄壤分别为3.04和3.75 kg·hm-2,占施用氮素的1.21%和1.50%.差异显著性检验表明,春季红壤与黄壤氨挥发通量差异达到比较显著水平(P<0.1),秋季基施和追施红壤和黄壤氨挥发通量差异均未达到显著水平.
  摘要[967]  PDF   HTML
家蚕凋亡相关基因ice的克隆及在大肠杆菌中的表达
  宋丽娜,王文兵,李兵,沈卫德  2007(2):270-273
ICE(白细胞介素-1β转换酶)是caspase家族 (半胱氨酸天门冬氨酸蛋白酶cys-teinylaspartate specific proteinase)中最早发现的成员,在多细胞生物细胞凋亡的过程中起着重要作用.为了深入了解模式昆虫家蚕ICE在凋亡通路中的作用,采用紫外线刺激家蚕细胞,PCR克隆获得2个新的ice基因不同剪切体,分别命名为ice-2和ice-5(GenBank登录号:DQ360829和DQ360830),并将这2个剪切体分别克隆进原核表达载体pET28a中,在大肠杆菌中诱导表达,对表达产物进行了检测,结果显示ICE-2和ICE-5蛋白表达过程中能发生自我剪切.
  摘要[794]  PDF   HTML
大豆异黄酮提取工艺条件的研究
  毛慧玲,彭娟,孙少发,黄鹃,欧阳涟  2007(2):274-278
对从大豆中提取大豆异黄酮的工艺进行了研究.在单因素试验结果与分析的基础上,通过正交试验,考察了料液比、乙醇浓度、提取次数和时间等因素对大豆异黄酮提取效果的影响.并根据试验结果,优化了大豆异黄酮的提取工艺条件,在1:7料液比、70%乙醇浸提、65℃恒温摇床提取2次、每次1 h的最优条件下,大豆异黄酮提取率可高达0.533%.
  摘要[709]  PDF   HTML
竹叶黄酮的提取纯化工艺及含量测定方法比较研究
  郭雪峰,岳永德  2007(2):279-282
对6个纯化竹叶黄酮工艺以及测量竹叶黄酮含量的分光光度法和高效液相色谱法进了比较研究.结果表明,工艺4聚酰胺柱吸附法为最佳工艺,纯化后竹叶黄酮浓度达0.59 mg·mL-1(分光光度法)和0.60 mg·mL-1(HPLC).两种方法测定的竹叶黄酮浓度值差异不大,结果稳定可靠.分光光度法操作简单方便,测定结果略微偏高, HPLC法重复性、稳定性均较好.
  摘要[786]  PDF   HTML
固定化醇氧化酶的制备和性质研究
  甘小平,张袖丽,吉秀锋  2007(2):283-286
以胺化纤维素为载体,固定醇氧化酶.应用分光光度法,找出了制备固定醇氧化酶的最佳反应条件,同时对固定化酶的性质进行测定.结果表明,固定化酶的最适反应pH值为7.4;最适反应温度为35℃,比溶液酶提高了10℃.米氏常数为13.8 mg·mL-1,略高于溶液酶(3.5 mg·mL-1).固定化酶表现出非常好的重复使用稳定性,重复使用9次后,剩余活力为70%.
  摘要[869]  PDF   HTML
东南沿海地区大麦白粉菌群体毒性分析
  李秀丽,朱靖环,陶跃之,高智谋,汪军妹,贾巧君,杨建明  2007(2):287-290
利用19个含有已知抗大麦白粉病基因的鉴别寄主,对采自福建莆田、江苏盐城、浙江海盐和杭州4个地区的213个大麦白粉病菌株,进行毒性基因鉴定和毒性频率分析.结果显示,在被调查的白粉菌群体中,尽管某些毒性基因的频率在不同地区有所不同,但大部分亚群体中毒性基因的频率在不同地区极为接近;在东南沿海4个地区大麦抗白粉病育种中,具有利用价值的抗病基因是:Mla11,Mlmw,Mlkb,Mlmlw,Mlk2,(Mla10 MlDu2)和(MlKl Mla8),无利用价值的抗病基因是:Mla8,Mlnn,Mlh,(Mlra Mla8),(MlRu2 Mla8),(MlBw Mla8),(Mlra MlDr2)和(MlLa Mla8).
  摘要[878]  PDF   HTML
安全多方计算的关键技术分析
  仲红  2007(2):291-295
安全多方计算(SMC)是研究分布式系统安全性的重要内容,对解决网络环境下的信息安全具有重要价值.作者概述了SMC的基本理论及应用研究进展,分析了SMC的基础协议及其相关的底层密码算法.对秘密分享、同态加密、零知识证明、茫然传输、秘密比较等SMC的关键技术进行了深入研究,提出值得进一步探讨的SMC热点问题.
  摘要[1109]  PDF   HTML
植酸酶对仔猪氮、磷排泄影响的研究
  陈文,黄艳群  2007(2):296-300
选用48头长白×荣昌杂交仔猪进行2个2×2因子的代谢试验,研究在高、低能量的仔猪饲粮添加植酸酶对氮、磷排泄的影响.每个试验均设2个有效磷水平(0.36%和0.26%)、2个植酸酶添加水平(0、750 FTU·kg-1),共4个处理组,每个处理组设6个重复,每个重复1头仔猪.试验1为高能水平(DE:14.3 MJ·kg-1),仔猪起始体重9.2 kg ;试验2为低能水平(DE:12.8 MJ·kg-1),仔猪起始体重16.9 kg.试验期2周.结果表明,添加植酸酶可明显降低粪中P、Ca、N百分含量和单位代谢体重N、Ca、P的排泄量,降低粪P含量及粪P排泄量都达显著或极显著水平,减少仔猪排泄物对环境的污染.
  摘要[780]  PDF   HTML
IMO和FOS对乳酸菌增殖的影响
  万荣峰,江善祥  2007(2):301-304
研究低聚异麦芽糖(IMO)和低聚果糖(FOS)对乳酸菌体外增殖和雏鸡盲肠内增殖的影响.结果显示,IMO和FOS均对乳酸菌有一定生长促进作用,培养液pH值略有下降,但与对照组相比,差异不显著(P>0.05);IMO和FOS可促进雏鸡盲肠乳酸菌的增殖、抑制大肠埃希菌的生长,在5 d后呈现差异显著性(P<0.05).
  摘要[751]  PDF   HTML

本期目录

《安徽农业大学学报》被收录情况
  2007(2):F0002
  摘要[441]  PDF   HTML

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