硒化修饰可以提高天然多糖的生物活性。以怀山药多糖（CYP）为原料，采用HNO3-Na2SeO3法制备怀山药硒多糖（Se-CYP），对其制备工艺和α-葡萄糖苷酶活性抑制作用进行了研究。单因素试验和正交试验结果表明，以反应产物的α-葡萄糖苷酶抑制率为指标时，最佳工艺条件为：CYP与Na2SeO3质量比为6:4，硝酸浓度为1.4%，反应时间为10 h，反应温度为80 ℃。相关性分析结果显示，Se-CYP的α-葡萄糖苷酶抑制率与其硒含量高度正相关。傅里叶红外光谱结果显示，硒以硒酯的方式存在于Se-CYP中，其存在形式主要为。不同浓度的Se-CYP均对α-葡萄糖苷酶活性表现出较强的抑制作用，且浓度越高，抑制率越高。相同浓度的Se-CYP的α-葡萄糖苷酶抑制率高于CYP。研究结果可以为筛选和制备高活性且绿色安全的α-葡萄糖苷酶抑制剂提供理论参考。
Selenization modification can improve the biological activity of natural polysaccharides. With Huaishan yam polysaccharide (CYP) as the raw material, selenium-polysaccharide from Huaishan yam (Se-CYP) was prepared by HNO3-Na2SeO3 method. The preparation technology and α-glucosidase activity inhibition of Se-CYP were studied. The results of single factor test and orthogonal test showed that, with the α-glucosidase inhibition rate as the index, the optimal conditions were as follows: mass ratio of CYP to Na2SeO3 was 6:4, concentration of nitric acid was 1.4%, reaction time was 10 h, and reaction temperature was 80 ℃. Correlation analysis showed that the α-glucosidase inhibition rate of Se-CYP was highly positively correlated with the selenium content. FTIR results showed that selenium existed in Se-CYP in the form of selenium ester, and its existence form was mainly as . Different concentrations of SE-CYP all showed strong inhibition on the α-glucosidase activity, and the higher the concentration, the higher the inhibition rate. The α-glucosidase inhibition rate of Se-CYP was higher than that of CYP at the same concentration. These results can provide a theoretical reference for screening and preparing of high activity and safe α-glucosidase inhibitors.